Monitoring the disease activity via the antibody-antigen recognition in paper

The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems Pub Date : 2013-04-07 DOI:10.1109/NEMS.2013.6559721

Hsi-Kai Wang, Cheng-Han Tsai, Chung-Tao Tang, Pi-Chun Li, J. Leou, Yin-Liang Tang, H. Hsieh, Han‐Chung Wu, Chao-Min Cheng

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引用次数: 2

Abstract

Dengue fever is one of the acute flavivrus-borne infectious diseases caused by dengue virus with four serotypes. To date, there are no efficient diagnostic tools available for monitoring the disease activity of dengue fever. It is needed to develop a diagnostic device with the characteristics of inexpensiveness, ease-to-use and robustness for the detection of dengue fever. In this study, we have developed an ELISA-based diagnostic device prepared via wax printing method through using filter paper. The antigens that we targeted to quantify in the buffer system were both non-structural protein 1 with the detection limit of about 100 pg/mL and envelope protein with the diluted virus culture soup of about 100 times of serotype 2 dengue virus (only 2 μL sample). We believe that this study would provide insight on the development of in-vitro diagnostic devices for dengue fever and various diseases in the different divisions of medicine.

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通过纸上抗体-抗原识别监测疾病活动

登革热是由登革热病毒引起的急性黄病毒传播传染病之一，有四种血清型。迄今为止，没有有效的诊断工具可用于监测登革热的疾病活动。需要研制一种价格低廉、使用方便、坚固耐用的登革热检测设备。在本研究中，我们开发了一种基于elisa的诊断装置，该装置是通过滤纸通过蜡印法制备的。我们在缓冲系统中定量的抗原为非结构蛋白1(检测限约为100 pg/mL)和包膜蛋白(约为2型登革热病毒的100倍稀释病毒培养汤(仅2 μL样品)。我们相信，这项研究将为登革热和不同医学部门的各种疾病的体外诊断设备的发展提供见解。

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The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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