Human biomonitoring of a urinary propetamphos metabolite using gas chromatography–mass spectrometry

Environmental and Occupational Health Practice Pub Date : 2021-07-14 DOI:10.1539/eohp.2020-0030-oa

N. C. Mohanto, Hirotaka Sato, Yuki Ito, J. Ueyama, Kyoko Minato, Naoko Oya, M. Miyake, Karin Nomasa, M. Gotoh, I. Saito, E. Shibata, M. Kamijima

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Abstract

Objectives: Propetamphos (PPT) is an organophosphate pesticide (OP) widely used to control insects in public health settings and methylethylphosphoramidothioate (MEPT) is a urinary exposure marker of PPT. The objectives of this study were to develop a biomonitoring method for urinary MEPT using gas chromatography–mass spectrometry (GC–MS) and to measure urinary MEPT concentrations in occupational and non-occupational human populations. Methods: Analytes derivatized with pentafluorobenzyl bromide were analyzed by GC–MS and dibu tyl phosphate was used as an internal standard. The validated method was applied to urine samples collected from occupational PPT sprayers (n =15), non-PPT sprayers (n=15) who did not spray PPT but sprayed other OPs, and control subjects (n=80) living in Aichi, Japan. Results: Calibration curves were obtained using standard-spiked pooled urine samples, and the coefficients of determination were ≥0.98. The limit of detection (LOD) was 10 μg/L. The within-run precision and between-run precision ranged from 17.5% to 19.4% and 10.4% to 18.1%, respectively. The detection rates of urinary MEPT in the PPT sprayers, non-PPT sprayers, and control subjects were 26.7%, 6.7%, and 2.5%, respectively. The concentration ranges for creatinine-unadjusted MEPT were

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用气相色谱-质谱法对人体尿中甲磷代谢物进行生物监测

目的:异丙磷(PPT)是一种广泛用于公共卫生控制昆虫的有机磷农药(OP)，甲基乙基磷酰胺硫代酸酯(MEPT)是异丙磷尿暴露标志物。本研究的目的是开发一种使用气相色谱-质谱(GC-MS)的尿液MEPT生物监测方法，并测量职业和非职业人群的尿液MEPT浓度。方法:以磷酸二丁酯为内标，采用气相色谱-质谱法对五氟苯溴衍生物进行分析。将验证方法应用于居住在日本爱知市的职业PPT喷雾器(n=15)、不喷PPT但喷其他OPs的非PPT喷雾器(n=15)和对照(n=80)的尿液样本。结果:用标准加标的合并尿样获得校准曲线，测定系数≥0.98。检出限为10 μg/L。运行内精密度为17.5% ~ 19.4%，运行间精密度为10.4% ~ 18.1%。尿MEPT在PPT喷雾器组、非PPT喷雾器组和对照组的检出率分别为26.7%、6.7%和2.5%。各组(PPT喷雾器、非PPT喷雾器和对照组)肌酐调节MEPT浓度范围分别为

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Environmental and Occupational Health Practice

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