Rohit Mishra, Glen Reilly, M. Agnew, Alexander Garvey, C. Rogers, Elaine Andrade, Hui Ma, Sean M. Fitzgerald, Julia Zapatero, R. O’Kennedy, J. Ducrée
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引用次数: 3
Abstract
Point-of-care diagnostics devices require user-friendly automation of multi-step / multi-reagent bioana-lytical protocols. We demonstrate, for the first time, Laser-actuated, Centrifugo-Pneumatic Valves (LCPVs) which open on-demand by ablating a remotely connected polymeric membrane with an instrument-based laser. Due to its small footprint, isolation of radiation-induced heating from the biological fluids and independence of radial position & contact-angle, the novel LCPV technology enables large-scale integration of flexibly customizable and operationally robust centrifugal microfluidic “Lab-on-a-Disc” (LoaD) systems. As a pilot application, we demonstrate the detection of Fatty Acid Binding Protein and Myeloperoxidase, both potential biomarkers used for cardiovascular disease (CVD) determination.
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