Assessment of Microbiological Quality Associated with Ready- to- Eat Bush Meat Sold at Rumuokoro Market in Rivers State

I. Ikeh, B. C. Anele, U. A. Ogbodo
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Abstract

The study was carried out to investigate the Microbiological quality of microorganisms associated with ready-to-eat bush meat sold at Rumuokoro market in Rivers state. Totally 24 samples were collected and analyzed using different media such as Nutrient agar for Total aerobic plate count (TAPC), MacConkey agar for the coliform count, Eosin methylene blue for Escherichia coli (EC), and Potato Dextrose Agar for Fungal count (FC) and ten (10) fold serial dilution was used. Staphylococcus spp, Pseudomonas spp, Bacillus spp, and Escherichia coli were isolated. The total aerobic plate count (TAPC), E. coli count (EC)-Coliform count (CC), and Fungal count (FC) isolated from antelope were higher when compared to grass-cutter so there was a significant difference (P <0.005). The occurrence of Staphylococcus aureus isolated from antelope (26.9%) was higher when compared to grass- cutter (25.0%). However the occurrence of Pseudomonas spp and Bacillus spp isolated from Antelope (23.1% and 30.8%) were higher when compared to grass- cutter (12.5% and 18.5%) while the occurrence of the above organisms isolated on both Antelope is significantly difference (P<0.005) from grass cutter. But the occurrence of Aspergillus spp and Penicillium spp were higher in grass cutter sample (57.1%) and (42.9%) compared to antelope (55.6%) and (44.4%) respectively, although the mean difference was statistically significant (P<0.005) so there was significant difference. It is hereby recommended that most handlers should always wash hands before and after handling the meat as improper hand washing is the number one cause of food borne illness. Consumers of such meat should learn food hygiene practices such as, soaking the meat in warm salt solution, proper washing and well cooked before consumption.
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河流州Rumuokoro市场出售的即食丛林肉的微生物质量评估
开展这项研究是为了调查河流州Rumuokoro市场销售的即食丛林肉相关微生物的微生物质量。共收集24份样品,使用不同培养基进行分析,如用于总需氧平板计数(TAPC)的营养琼脂,用于大肠菌群计数的麦康基琼脂,用于大肠杆菌计数(EC)的伊红亚甲基蓝,用于真菌计数(FC)的马铃薯葡萄糖琼脂,并使用10倍连续稀释。分离出葡萄球菌、假单胞菌、芽孢杆菌和大肠杆菌。总需氧平板计数(TAPC)、大肠杆菌计数(EC)-大肠菌群计数(CC)和真菌计数(FC)均高于草畜,差异有统计学意义(P <0.005)。从羚羊中分离出的金黄色葡萄球菌(26.9%)高于从割草动物中分离出的金黄色葡萄球菌(25.0%)。而从羚羊身上分离出的假单胞菌和芽孢杆菌的检出率分别为23.1%和30.8%,高于割草动物(12.5%和18.5%),而从割草动物身上分离出的两种细菌检出率均显著高于割草动物(P<0.005)。但割草者中曲霉菌和青霉菌的发生率分别为57.1%和42.9%,高于羚羊(55.6%)和(44.4%),但平均差异有统计学意义(P<0.005),差异有统计学意义。因此,我们建议大多数处理人员在处理肉类之前和之后都要洗手,因为不正确的洗手是导致食源性疾病的头号原因。食用这类肉类的消费者应学习食物卫生习惯,例如在食用前将肉浸在温盐溶液中、适当洗净及煮熟。
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