A Kinetic Study for In-vitro Intestinal Uptake of Monosaccharide across Rat Everted Gut Sacs In the Presence of Some Antidiabetic Medicinal Plants

Abstract

Enicostemma hyssopifolium (EH), Eugenia jumbolana (EJ), Tinospora cordifolia (TC), Gymnema sylvestre (GS), and Trigonella foenum (TF) are traditional antidiabetic medicinal plant used in India. An everted rat gut sac technique was used to investigate the effect of aqueous extracts of these plants on kinetic parameters of D (+) glucose. Everted guts were mounted in a gut sac bath and aqueous extracts were added to the mucosal medium at varying substrate concentrations. Michaelis-Menten constant (Km) and maximal velocity (Vmax) were calculated in the presence and absence of drug. It was observed that aqueous extract of EJ, TC, GS and TF significantly reduced Vmax of D (+)-glucose uptake by 40.09, 28.67, 83.67 and 34.5 μM hr -1 respectively, whereas Km remained unaltered suggested a non-competitive type of inhibition was present.