Bacterial Contamination of Escherichia coli in Long Beans at Traditional Markets

M. A. Shodikin, I. Sutejo, D. Agustina, A. Raharjo, Anaditya Wahyu Kumudhaningsih
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引用次数: 1

Abstract

Escherichia coli (E. coli) bacteria may contaminating uncooked food and cause food poisoning disease. Long beans (Vigna sinensis L) is one of the vegetable that consumed by people in raw (uncooked) and often traded in traditional markets. Many traditional markets do not meet sanitation requirements and many sellers do not perform good personal hygiene. These situation can potentially to cause bacterial contamination of vegetables sold in traditional markets. There has been no research examining E. coli contamination in long beans that sold at traditional markets in Jember.The purpose of this study to prove the contamination of E. coli bacteria in long beans at traditional markets in Jember. The research method uses an observational descriptive design with the cross sectional approach. Research places in eight traditional markets in Jember Regency and Microbiology Laboratory, Faculty of Medicine, University of Jember. The samples used amounted to 40 long beans purchased from 40 traditional market traders. The microbiological examination is performed using the most probable number (MPN) method, namely presumptive test and completed test. The results showed from the presumptive test all of 40 samples (100%) contaminated with coliform bacteria. After completed tests, 36 out of 40 samples (90%) were found contaminated by E. coli. The majority of long beans sold in traditional markets have been contaminated by E. coli bacteria.
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传统街市长豆的大肠杆菌污染情况
大肠杆菌可能污染未煮熟的食物,引起食物中毒。长豆(Vigna sinensis L)是人们生吃(未煮熟)的蔬菜之一,经常在传统市场上交易。许多传统市场不符合卫生要求,许多卖主不讲个人卫生。这些情况可能会导致传统市场上出售的蔬菜受到细菌污染。目前还没有对去年12月在传统市场销售的长豆中大肠杆菌污染的研究。本研究的目的是为了证明在传统市场的长豆大肠杆菌的污染。研究方法采用观察性描述性设计和横断面方法。研究地点在Jember县的八个传统市场和Jember大学医学院微生物实验室。使用的样品从40个传统市场商人那里购买了40个长豆。微生物学检查采用最可能数(MPN)法,即假定检验和完成检验。推定检测结果显示,40份样品全部(100%)被大肠菌群污染。在完成测试后,40个样本中有36个(90%)被大肠杆菌污染。传统市场上出售的大部分长豆都被大肠杆菌污染了。
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