Infranatant Portion of Microfragmented Adipose Tissue: A Promising Source of SVF for the Management of Androgenetic Alopecia

Abstract

Aim: The purpose of this article is to prove the possibility to transfer a good amount of cells of the SVF (and ADSCs) in the infranatant portion of microfragmented adipose tissue. Method: The adipose tissue harvesting procedure is performed under local anaesthesia. The adipose tissue was harvested with a 2 mm diameter microperforated cannula with 1 mm side port holes, mounted inside the special patented guide. Both cannula and guide are included in the SEFFIHAIR™ medical device. Once the adipose tissue is harvested, it is gently washed, and it was divided in two specimens: (EMU) the tissue was emulsified with 20 passages from one syringe to another and (CTRL) the tissue didn’t undergo any emulsification. Results: The emulsification procedure liberated alive and proliferating cells and we observed that the specimens derived with a 1 mm side port hole cannula and then emulsified (EMU) showed a higher number of cells in the infranatant part compared to the one derived from the control tissue without any (1 EMU vs. 1EMU infra). Conclusion: The use of microcannulas, in combination with a mechanical digestion by an emulsification procedure and centrifuge, could ease SVF cells isolation for regenerative treatment and could also be performed in a medical facility.