Pub Date : 2023-09-06DOI: 10.31487/j.rgm.2023.02.02
J. Alzeer
The article explores the integration of duality and homeostasis in healthcare to enhance health outcomes. It emphasizes the importance of maintaining balance and stability in both physical and non-physical aspects of the body for optimal well-being. The rationalization of the duality concept is discussed, drawing analogies from various natural systems and phenomena. The role of duality in homeostasis, including mental stability, metabolic processes, and energy forms, is explored. The article also highlights the significance of embracing duality in personal balance, lifestyle choices, and immune system activation. Furthermore, it discusses the implications of incorporating duality in disease prevention and treatment, emphasizing the integration of physical and non-physical aspects in personalized medicine. The synergy between medicine and lifestyle is highlighted in the context of lifestylopathy, emphasizing the need for compatibility and personalized approaches. Overall, the article concludes by highlighting the potential of embracing duality and homeostasis to revolutionize healthcare and improve patient outcomes.
{"title":"Lifestylopathy: Unlocking Potential by Embracing Duality and Homeostasis for Improved Healthcare","authors":"J. Alzeer","doi":"10.31487/j.rgm.2023.02.02","DOIUrl":"https://doi.org/10.31487/j.rgm.2023.02.02","url":null,"abstract":"The article explores the integration of duality and homeostasis in healthcare to enhance health outcomes. It emphasizes the importance of maintaining balance and stability in both physical and non-physical aspects of the body for optimal well-being. The rationalization of the duality concept is discussed, drawing analogies from various natural systems and phenomena. The role of duality in homeostasis, including mental stability, metabolic processes, and energy forms, is explored. The article also highlights the significance of embracing duality in personal balance, lifestyle choices, and immune system activation. Furthermore, it discusses the implications of incorporating duality in disease prevention and treatment, emphasizing the integration of physical and non-physical aspects in personalized medicine. The synergy between medicine and lifestyle is highlighted in the context of lifestylopathy, emphasizing the need for compatibility and personalized approaches. Overall, the article concludes by highlighting the potential of embracing duality and homeostasis to revolutionize healthcare and improve patient outcomes.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115475171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-13DOI: 10.31487/j.rgm.2023.02.01
M. Wheeler, Aaron J. Maki, R. A. Chanaka Rabel
This study was designed to develop a fibrin scaffold for optimum in vitro osteogenic differentiation of porcine ASCs. Fibrin scaffolds physically and chemically modified to be stiffer or have higher concentrations of calcium and phosphate ions were hypothesized to enhance osteogenic differentiation compared to control fibrin scaffolds. Treatments during coagulation resulted in six scaffold types for comparison: whole blood controls, red blood cell lysis buffer, calcium chloride, calcium hydrogen phosphate, vacuum, and mechanical compression. Based on the results, fibrin supplemented with granules of calcium hydrogen phosphate (CaHPO4) was determined to be the most suitable formulation of those examined. Using calcium phosphate resulted in a fibrin scaffold that coagulated faster (p = 0.022), had a rougher surface, higher stiffness, and desirable properties for practical use during surgical operations and scaffolds used in bone tissue engineering. Further, osteogenic differentiation was enhanced on scaffolds treated with calcium phosphate. In addition, fibrin scaffolds treated with RBC lysis buffer were also stiffer than untreated controls. These results are partly explained by ASC attachment and fibrin polymerization during coagulation.
{"title":"Calcium Phosphate Treatment Enhances Osteogenic Differentiation of Porcine Adipose-Derived Stem Cells on Fibrin Scaffolds","authors":"M. Wheeler, Aaron J. Maki, R. A. Chanaka Rabel","doi":"10.31487/j.rgm.2023.02.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2023.02.01","url":null,"abstract":"This study was designed to develop a fibrin scaffold for optimum in vitro osteogenic differentiation of porcine ASCs. Fibrin scaffolds physically and chemically modified to be stiffer or have higher concentrations of calcium and phosphate ions were hypothesized to enhance osteogenic differentiation compared to control fibrin scaffolds. Treatments during coagulation resulted in six scaffold types for comparison: whole blood controls, red blood cell lysis buffer, calcium chloride, calcium hydrogen phosphate, vacuum, and mechanical compression. Based on the results, fibrin supplemented with granules of calcium hydrogen phosphate (CaHPO4) was determined to be the most suitable formulation of those examined. Using calcium phosphate resulted in a fibrin scaffold that coagulated faster (p = 0.022), had a rougher surface, higher stiffness, and desirable properties for practical use during surgical operations and scaffolds used in bone tissue engineering. Further, osteogenic differentiation was enhanced on scaffolds treated with calcium phosphate. In addition, fibrin scaffolds treated with RBC lysis buffer were also stiffer than untreated controls. These results are partly explained by ASC attachment and fibrin polymerization during coagulation.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122713660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-10DOI: 10.31487/j.rgm.2023.01.01
M. Wheeler, Aaron J. Maki, R. A. Chanaka Rabel
The proliferation and migration of porcine adipose-derived stem cells (ASCs) have only begun to be studied in detail in vitro. The primary aim of these studies was to analyse in vitro ASC migration in varying concentrations of growth factors derived from the platelet-rich fraction of centrifuged plasma, the so-called platelet-rich plasma (PRP). ASCs were hypothesized to migrate at a faster velocity with increasing PRP concentrations. Whole blood was collected with a sodium citrate anticoagulant and subjected to two centrifugations. The first slower spin was to remove red blood cells to collect plasma, and the second faster spin was to collect the fraction with a high platelet concentration. Platelet concentration increased 3.5-fold compared to plasma, within the prescribed therapeutic range. For long-term culture of ASCs, DMEM supplemented with no more than 20% was necessary to maintain cell viability. ASC proliferation rate in PRP-supplemented media was comparable to that in fetal bovine serum (FBS), a standard cell culture media supplement. In addition, the velocity of ASC migration increased in cultures supplemented with 10%, 20% or 30% PRP. Generally, PRP was determined to be a media supplement with similar effects as FBS, potentially making it a suitable substitute for in vitro expansion of ASC populations. These results are likely partly explained by similarities in growth factor concentrations and their effects. Further characterization of PRP will be necessary to tease out the specific growth factor(s) responsible for the increase in swine ASC migration.
{"title":"In Vitro Migration of Porcine Adipose-Derived Stem Cells in Platelet-Rich Plasma","authors":"M. Wheeler, Aaron J. Maki, R. A. Chanaka Rabel","doi":"10.31487/j.rgm.2023.01.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2023.01.01","url":null,"abstract":"The proliferation and migration of porcine adipose-derived stem cells (ASCs) have only begun to be studied in detail in vitro. The primary aim of these studies was to analyse in vitro ASC migration in varying concentrations of growth factors derived from the platelet-rich fraction of centrifuged plasma, the so-called platelet-rich plasma (PRP). ASCs were hypothesized to migrate at a faster velocity with increasing PRP concentrations. Whole blood was collected with a sodium citrate anticoagulant and subjected to two centrifugations. The first slower spin was to remove red blood cells to collect plasma, and the second faster spin was to collect the fraction with a high platelet concentration. Platelet concentration increased 3.5-fold compared to plasma, within the prescribed therapeutic range. For long-term culture of ASCs, DMEM supplemented with no more than 20% was necessary to maintain cell viability. ASC proliferation rate in PRP-supplemented media was comparable to that in fetal bovine serum (FBS), a standard cell culture media supplement. In addition, the velocity of ASC migration increased in cultures supplemented with 10%, 20% or 30% PRP. Generally, PRP was determined to be a media supplement with similar effects as FBS, potentially making it a suitable substitute for in vitro expansion of ASC populations. These results are likely partly explained by similarities in growth factor concentrations and their effects. Further characterization of PRP will be necessary to tease out the specific growth factor(s) responsible for the increase in swine ASC migration.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130391514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.31487/j.rgm.2022.02.06
J. Alzeer
Chemical and biochemical reactions are carried out either to generate energy or to produce useful macromolecules. Entropy is a well-applied concept in many fields, including physics, chemistry, biology, and medicine. Various perspectives have been used to describe the concept, creating confusion and misconceptions. In chemical and biochemical reactions, entropy plays a significant role in the directionality and spontaneity of the reactions. Potential energy can be used to better understand the concept of entropy. Potential energy represents order, while entropy represents disorder; therefore, they are inversely proportional and intimately linked. Molecules with high potential usually have rich sets of functions and information, which is due to the enrichment of their constitutions, configurations, and conformations. In molecules with low potential, there are greater vibrational, rotational, and translational motions associated with decreased order in their constitution, configuration, and conformation. Distribution of electronic charge changes in macromolecules over time, increasing the rotation of side-chain residues and thus increasing entropy and affecting potential in terms of structure, function, and information. Entropy can thus be defined as a state of spontaneous change, bound to time and constantly increasing, which causes structural changes in the form of constitution, configuration, and conformation, and functional changes in the form of the ability to do work as well as informational changes in the form of the transmission of commands.
{"title":"Directionality of Chemical Reaction and Spontaneity of Biological Process in the Context of Entropy","authors":"J. Alzeer","doi":"10.31487/j.rgm.2022.02.06","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.02.06","url":null,"abstract":"Chemical and biochemical reactions are carried out either to generate energy or to produce useful macromolecules. Entropy is a well-applied concept in many fields, including physics, chemistry, biology, and medicine. Various perspectives have been used to describe the concept, creating confusion and misconceptions. In chemical and biochemical reactions, entropy plays a significant role in the directionality and spontaneity of the reactions. Potential energy can be used to better understand the concept of entropy. Potential energy represents order, while entropy represents disorder; therefore, they are inversely proportional and intimately linked. Molecules with high potential usually have rich sets of functions and information, which is due to the enrichment of their constitutions, configurations, and conformations. In molecules with low potential, there are greater vibrational, rotational, and translational motions associated with decreased order in their constitution, configuration, and conformation. Distribution of electronic charge changes in macromolecules over time, increasing the rotation of side-chain residues and thus increasing entropy and affecting potential in terms of structure, function, and information. Entropy can thus be defined as a state of spontaneous change, bound to time and constantly increasing, which causes structural changes in the form of constitution, configuration, and conformation, and functional changes in the form of the ability to do work as well as informational changes in the form of the transmission of commands.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"201 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116168063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.31487/j.rgm.2022.02.05
M. De Monti, Laura De Pellegrin, K. Galetti
Chronic cutaneous non-healing fistulas very often are dehiscences of surgical or traumatic wounds that do not repair properly and progressively undergo intrafistular and perifistular fibrosis. The fibrous tissue constitutes a natural barrier to the progression of the fistula repair process and represents the major cause of non-healing and chronicization even in a context of proper vascularization. The microfractured autologous adipose graft allows to provide the tissues involved in the fibrotic process with a regenerative stimulus by MSCs (mesenchymal stem cells) contained in adipose clusters of 0.3 mm. In this context, MSCs are able to secrete cytokines with antibiotic, antifibrotic, angiogenic and analgesic effects. The micro-fragmentation technique guarantees a high regenerative effect, as the MSCs are not isolated enzymatically with the simultaneous destruction of the adipose tissue. The micro-fragmentation allows the maintainance of the structure of the adipose cluster including microvessels and allows it to amplify by 6000 times the active surface that exposes the MSCs. Our experience with the mechanical microfracturing method of lipoaspirate consists of 41 treatments. In 7 cases the graft was performed due to the presence of a non-healing cutaneous fistula, which lasted from 128 to 243 days. In 6 cases we achieved immediate repair and closure of the fistula while in one case the procedure failed. The purpose of the paper is to describe in detail our experience by an accurate description of the implemented method of the used device accompanied by the document with adequate photographic documentation.
{"title":"Microfractured Adipose Tissue Graft for the Advanced Treatment of Non-Healing Cutaneous Fistulas","authors":"M. De Monti, Laura De Pellegrin, K. Galetti","doi":"10.31487/j.rgm.2022.02.05","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.02.05","url":null,"abstract":"Chronic cutaneous non-healing fistulas very often are dehiscences of surgical or traumatic wounds that do not repair properly and progressively undergo intrafistular and perifistular fibrosis. The fibrous tissue constitutes a natural barrier to the progression of the fistula repair process and represents the major cause of non-healing and chronicization even in a context of proper vascularization. The microfractured autologous adipose graft allows to provide the tissues involved in the fibrotic process with a regenerative stimulus by MSCs (mesenchymal stem cells) contained in adipose clusters of 0.3 mm. In this context, MSCs are able to secrete cytokines with antibiotic, antifibrotic, angiogenic and analgesic effects. The micro-fragmentation technique guarantees a high regenerative effect, as the MSCs are not isolated enzymatically with the simultaneous destruction of the adipose tissue. The micro-fragmentation allows the maintainance of the structure of the adipose cluster including microvessels and allows it to amplify by 6000 times the active surface that exposes the MSCs. Our experience with the mechanical microfracturing method of lipoaspirate consists of 41 treatments. In 7 cases the graft was performed due to the presence of a non-healing cutaneous fistula, which lasted from 128 to 243 days. In 6 cases we achieved immediate repair and closure of the fistula while in one case the procedure failed. The purpose of the paper is to describe in detail our experience by an accurate description of the implemented method of the used device accompanied by the document with adequate photographic documentation.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125422334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-14DOI: 10.31487/j.rgm.2022.02.02
M. Benakli, Redhouane Ahmed Nacer, F. Mehdid, Mounira Baazizi, N. Rahmoune, Dina Ait Ouali, Hanane Bouarab, Sara Zerkout, Fouzia Louar, Rose Marie Hamladji
Juvenile myelomonocytic leukemia (JMML) is a rare hematological malignancy of early childhood, classified by the World Health Organization as a myelodysplastic/myeloproliferative disease and is associated with a poor prognosis. Allogeneic hematopoietic stem cell transplantation is the only curative treatment. A two-year-old male child was diagnosed with JMML and was given induction chemotherapy. One year after diagnosis, the patient received allogeneic hematopoietic stem cell transplantation from an HLA sibling donor after a myeloablative conditioning regimen. The patient remained free of disease after 5 years of follow-up, healthy, with complete clinical, immunologic and hematologic recovery, without signs of JMML. Transplantation is the only modality to achieve a cure in JMML patients. The most widely practiced approach is the use of bone marrow or peripheral blood stem cells after a myeloablative conditioning regimen. Post-transplant monitoring chimerism can help identify the patients who are at risk of relapse.
{"title":"Hematopoietic Stem Cell Transplantation in Juvenile Myelomonocytic Leukemia: A Case Report and Literature Review","authors":"M. Benakli, Redhouane Ahmed Nacer, F. Mehdid, Mounira Baazizi, N. Rahmoune, Dina Ait Ouali, Hanane Bouarab, Sara Zerkout, Fouzia Louar, Rose Marie Hamladji","doi":"10.31487/j.rgm.2022.02.02","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.02.02","url":null,"abstract":"Juvenile myelomonocytic leukemia (JMML) is a rare hematological malignancy of early childhood, classified by the World Health Organization as a myelodysplastic/myeloproliferative disease and is associated with a poor prognosis. Allogeneic hematopoietic stem cell transplantation is the only curative treatment. A two-year-old male child was diagnosed with JMML and was given induction chemotherapy. One year after diagnosis, the patient received allogeneic hematopoietic stem cell transplantation from an HLA sibling donor after a myeloablative conditioning regimen. The patient remained free of disease after 5 years of follow-up, healthy, with complete clinical, immunologic and hematologic recovery, without signs of JMML. Transplantation is the only modality to achieve a cure in JMML patients. The most widely practiced approach is the use of bone marrow or peripheral blood stem cells after a myeloablative conditioning regimen. Post-transplant monitoring chimerism can help identify the patients who are at risk of relapse.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131840921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-07DOI: 10.31487/j.rgm.2022.02.03
M. De Monti, G. Cestaro, L. Regusci, F. Fasolini, K. Galetti
Background: Recurrent anal fistulas present a challenge to surgeons due to the high risk of post-operative incontinence caused by repeated surgery. The correct identification of the anatomy of the main and secondary fistula tracts and the individuation of abscess cavities are fundamental for correct treatment. Intraoperative endoscopic evaluation and the complete destruction of the fistula pathway can be achieved through video-assisted anal fistula treatment (VAAFT). Furthermore, the injection of human autologous Microfractured Adipose Tissue (MFAT) processed by a Lipogems® device can be used as both a bulking agent and a regenerative technique. Methods: A combined approach of VAAFT plus Microfractured Adipose Tissue Graft (MFAT) is proposed in order to treat recurrent and complex fistula in ano. Results: Three cases treated with a combination of VAAFT and MFAT grafts are described. All cases had undergone multiple interventions at the perianal level over a period ranging from 1 to 15 years. One case certainly failed due to poor patient compliance, but in the remaining two cases, the patients made a complete recovery with the disappearance of symptoms over a follow-up period of one to two years. Conclusion: The combination of video-assisted anal fistula treatment and injection of human autologous microfractured adipose tissue may be a valid, safe and feasible therapeutic option. MFAT injections are more effective in promoting tissue regeneration than simply “filling” the fistula tract and are common practice also in the treatment of Crohn’s Disease due to the immunomodulatory power of mesenchymal cells.
{"title":"Regenerative and Endoscopic Treatment of Complex Recurrent Fistula in Ano: When Technology Supports Clinical Treatment","authors":"M. De Monti, G. Cestaro, L. Regusci, F. Fasolini, K. Galetti","doi":"10.31487/j.rgm.2022.02.03","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.02.03","url":null,"abstract":"Background: Recurrent anal fistulas present a challenge to surgeons due to the high risk of post-operative incontinence caused by repeated surgery. The correct identification of the anatomy of the main and secondary fistula tracts and the individuation of abscess cavities are fundamental for correct treatment. Intraoperative endoscopic evaluation and the complete destruction of the fistula pathway can be achieved through video-assisted anal fistula treatment (VAAFT). Furthermore, the injection of human autologous Microfractured Adipose Tissue (MFAT) processed by a Lipogems® device can be used as both a bulking agent and a regenerative technique.\u0000Methods: A combined approach of VAAFT plus Microfractured Adipose Tissue Graft (MFAT) is proposed in order to treat recurrent and complex fistula in ano.\u0000Results: Three cases treated with a combination of VAAFT and MFAT grafts are described. All cases had undergone multiple interventions at the perianal level over a period ranging from 1 to 15 years. One case certainly failed due to poor patient compliance, but in the remaining two cases, the patients made a complete recovery with the disappearance of symptoms over a follow-up period of one to two years.\u0000Conclusion: The combination of video-assisted anal fistula treatment and injection of human autologous microfractured adipose tissue may be a valid, safe and feasible therapeutic option. MFAT injections are more effective in promoting tissue regeneration than simply “filling” the fistula tract and are common practice also in the treatment of Crohn’s Disease due to the immunomodulatory power of mesenchymal cells.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127710215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-16DOI: 10.31487/j.rgm.2022.02.01
D. Nagore, S. Pandya, Chetan Savaliya, Kamlesh Thummar
Type I or Type II diabetes, once recognized as juvenile diabetes or insulin-dependent diabetes, is a chronic illness in which the pancreas produces slight or no insulin. Insulin-dependent diabetes is a syndrome of glucose homeostasis considered by autoimmune destruction of the insulin-producing pancreatic Beta-cell that gradually leads to insulin deficiency. As there is no perfect treatment for management, Gplife has thought of and developed a product that is found to be effective in the management of insulin-dependent diabetes. After 60 days of evaluation of cases, it is observed that fasting blood glucose was reduced by 62.25%, postprandial glucose levels were reduced by 62.37%, HBA1C levels were reduced by 39.57%, C Peptide levels were increased by 26.67%, also the external insulin injection requirement of the patient’s decreased by 88.57%. This case study gives an overview of the current understanding of the disease and the efficacy of advance diabetic support products in insulin-dependent diabetes. It is evident that the said product further helps to reduce insulin and OHA doses for the management of insulin-dependent diabetes. It is suggested that the advance diabetes support product should be further extensively used as a monotherapy or adjunctive therapy for the regulation, or management or control of insulin-dependent diabetes.
{"title":"Pharmacological Potential of Advance Diabetes Support Product in the Management of Insulin-Dependent Diabetes: Overviews on Case Study","authors":"D. Nagore, S. Pandya, Chetan Savaliya, Kamlesh Thummar","doi":"10.31487/j.rgm.2022.02.01","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.02.01","url":null,"abstract":"Type I or Type II diabetes, once recognized as juvenile diabetes or insulin-dependent diabetes, is a chronic illness in which the pancreas produces slight or no insulin. Insulin-dependent diabetes is a syndrome of glucose homeostasis considered by autoimmune destruction of the insulin-producing pancreatic Beta-cell that gradually leads to insulin deficiency. As there is no perfect treatment for management, Gplife has thought of and developed a product that is found to be effective in the management of insulin-dependent diabetes. After 60 days of evaluation of cases, it is observed that fasting blood glucose was reduced by 62.25%, postprandial glucose levels were reduced by 62.37%, HBA1C levels were reduced by 39.57%, C Peptide levels were increased by 26.67%, also the external insulin injection requirement of the patient’s decreased by 88.57%. This case study gives an overview of the current understanding of the disease and the efficacy of advance diabetic support products in insulin-dependent diabetes. It is evident that the said product further helps to reduce insulin and OHA doses for the management of insulin-dependent diabetes. It is suggested that the advance diabetes support product should be further extensively used as a monotherapy or adjunctive therapy for the regulation, or management or control of insulin-dependent diabetes.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132545194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-18DOI: 10.31487/j.rgm.2022.01.03
D. Nagore, S. Pandya, Chetan Savaliya, Kamlesh Thummar, V. Undale
Aim: Diabetes support product (ADSP) with phytocompounds was proposed to improve insulin secretion, avoid pancreatic beta cell apoptosis, and moderate beta cell differentiation and proliferation. In this research work, beta cell regenerative potential of ADSP was evaluated with STZ induced diabetes in rodents. Method: Single dose of Streptozotocin (STZ) (70mpk; i.p.) was used to induce diabetes in the Wistar rats. The treatment of vehicle or test or GLB was continued for the next 28 days to assess sub-acute anti-diabetic potential. Fasting blood glucose levels (BGL) was monitored weekly once throughout the experiment. Bodyweight, Feed-water consumption was calculated on every day till end of the experiment. Interleukin-6, Interleukin-1β and Interferon-γ was also analysis from blood serum after 28 days of treatment in rats. Further, animal was humanely sacrifice and organs such as liver, kidney(s) and pancreas were isolated for histopathology analysis. Result: Research showed that Insulin is Increased by 3 times in comparison with the diabetic group by ADSP after 28 days. After 28 days treatment of ADSP to rodents, Interleukin-6 decreased by 52%, Interleukin-1β decreased by 73% and Interferon-γ decreased by 28% in comparison with the diabetic control group. It is also observed in histopathology studies that there was a rise in the quantity of islets after 28 days treatment of ADSP in Streptozotocin carried diabetic rats. Conclusion: Hence in conclusion, advance diabetes support product supposed to be appreciated as synergistic product of encouraging the β-cell regeneration in vivo.
{"title":"Evaluation of the Effect of an Advance Diabetes Support Product Targeting the Improved β-Cell Functions and β-Cell Regeneration for the Management of Diabetes Mellitus","authors":"D. Nagore, S. Pandya, Chetan Savaliya, Kamlesh Thummar, V. Undale","doi":"10.31487/j.rgm.2022.01.03","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.01.03","url":null,"abstract":"Aim: Diabetes support product (ADSP) with phytocompounds was proposed to improve insulin secretion, avoid pancreatic beta cell apoptosis, and moderate beta cell differentiation and proliferation. In this research work, beta cell regenerative potential of ADSP was evaluated with STZ induced diabetes in rodents.\u0000Method: Single dose of Streptozotocin (STZ) (70mpk; i.p.) was used to induce diabetes in the Wistar rats. The treatment of vehicle or test or GLB was continued for the next 28 days to assess sub-acute anti-diabetic potential. Fasting blood glucose levels (BGL) was monitored weekly once throughout the experiment. Bodyweight, Feed-water consumption was calculated on every day till end of the experiment. Interleukin-6, Interleukin-1β and Interferon-γ was also analysis from blood serum after 28 days of treatment in rats. Further, animal was humanely sacrifice and organs such as liver, kidney(s) and pancreas were isolated for histopathology analysis. \u0000Result: Research showed that Insulin is Increased by 3 times in comparison with the diabetic group by ADSP after 28 days. After 28 days treatment of ADSP to rodents, Interleukin-6 decreased by 52%, Interleukin-1β decreased by 73% and Interferon-γ decreased by 28% in comparison with the diabetic control group. It is also observed in histopathology studies that there was a rise in the quantity of islets after 28 days treatment of ADSP in Streptozotocin carried diabetic rats. \u0000Conclusion: Hence in conclusion, advance diabetes support product supposed to be appreciated as synergistic product of encouraging the β-cell regeneration in vivo.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116291723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-25DOI: 10.31487/j.rgm.2022.01.02
J. Alzeer
The human mind is highly exposed to different sources of information. According to halalopathy, supportive information enriches potential, while distracting information increases entropy. Potential energy and entropy are inversely proportional. The enrichment of potential energy creates favourable circumstances for prevention and recovery, while the accumulation of entropy accelerates aging and the development of diseases. The availability of potential energy plays a crucial role in how the immune system can exist and behave. Fight mode i.e., high potential energy mode where errors and defects are detected and corrected simultaneously. Fright mode in which potential energy is dispersed and entropy is accelerated, causing the immune system to respond randomly and attack indiscriminately. The flight mode, in which potential energy is suppressed, leads to a slowing down of the immune response, thereby bypassing many defects and mutations. The main objective of halalopathy is to develop methods and strategies to enrich the potential, keep the fight mode active and thus stimulate the immune system for better prevention and recovery.
{"title":"Halalopathy: Stimulation of the Immune System Through Enrichment of Potential Energy","authors":"J. Alzeer","doi":"10.31487/j.rgm.2022.01.02","DOIUrl":"https://doi.org/10.31487/j.rgm.2022.01.02","url":null,"abstract":"The human mind is highly exposed to different sources of information. According to halalopathy, supportive information enriches potential, while distracting information increases entropy. Potential energy and entropy are inversely proportional. The enrichment of potential energy creates favourable circumstances for prevention and recovery, while the accumulation of entropy accelerates aging and the development of diseases. The availability of potential energy plays a crucial role in how the immune system can exist and behave. Fight mode i.e., high potential energy mode where errors and defects are detected and corrected simultaneously. Fright mode in which potential energy is dispersed and entropy is accelerated, causing the immune system to respond randomly and attack indiscriminately. The flight mode, in which potential energy is suppressed, leads to a slowing down of the immune response, thereby bypassing many defects and mutations. The main objective of halalopathy is to develop methods and strategies to enrich the potential, keep the fight mode active and thus stimulate the immune system for better prevention and recovery.","PeriodicalId":148803,"journal":{"name":"International Journal of Regenerative Medicine","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124147634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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