Three dimensional lobule-mimetic regeneration in vitro by PEGDA-based cell sheets

Abstract

We propose a forming method of hydrogel-based cell sheets for regenerating three dimensional lobule-mimetic constructions. Accumulating the cells are conventional methods of three dimensional designs. In this study, we have designed a cell sheet chip to form a poly (ethylene glycol) diacrylate (PEGDA)-based cell sheet by two methods. First method is to pattern cells by DEP force for mimicking the pattern of the hepatocyte in vivo. Second method is to embed cells by UV light for photopolymerizing the PEGDA-based cell sheet. Stacking chip was used to form multilayer PEGDA-based cell sheets. The three dimensional lobule-mimetic regeneration will have widespread the applications in tissue engineering and detoxication experiments.