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2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII)最新文献

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The influence of molecular charges on microtubule curvatures in an electrical field 电场中分子电荷对微管曲率的影响
N. Isozaki, T. Nakahara, S. Ando, N. K. Kamisetty, H. Shintaku, H. Kotera, E. Meyhofer, R. Yokokawa
Toward a novel molecular separation, we propose a method to control gliding directions of microtubules (MTs) using a kinesin-MT system. The directions are controlled by electrical charges of cargo molecules partially labeled on the minus ends of MTs. We designed MTs labeled with streptavidin (SA), nanogold (NG), or dsDNA molecules, and evaluated their curvatures in an electrical field. The curvature of DNA-MT was larger than those of SA-MT or NG-MT because of DNA's large negative charge. These differences of curvatures enable to separate gliding MTs according to their cargo molecules.
为了实现一种新的分子分离,我们提出了一种利用驱动蛋白-微管系统控制微管滑动方向的方法。这些方向是由部分标记在MTs负端的载货分子的电荷控制的。我们设计了用链霉亲和素(SA)、纳米金(NG)或dsDNA分子标记的MTs,并评估了它们在电场中的曲率。DNA- mt的曲率比SA-MT和NG-MT的曲率大,因为DNA的负电荷较大。这些曲率的差异使我们能够根据它们的货物分子来分离滑行mt。
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引用次数: 0
Droplet bisulfite conversion platform for epigenetic cancer biomarker detection 亚硫酸氢盐液滴转化平台用于表观遗传癌症生物标志物检测
D. J. Shin, A. Stark, T. H. Wang
We report an integrated digital microfluidic platform for methylation biological samples. Recent advances in epigenetic studies highlight the importance of DNA methylation in the biology of cancer. The epigenetic signature of promoter regions in the genes ADAMTS1 and BNC1 has been shown to have strong correlation with the early stages of pancreatic carcinogenesis [1]. Most analytical techniques for studying methylation require pre-processing of sample DNA via bisulfite conversion. However, repetitive addition and removal of liquid reagents using conventional tubes and pipettes present a technical hurdle for microliter-scale reagents, as well as a high risk for contamination and human error. Previously, we reported a magnetically actuated platform for integrated DNA extraction and amplification from crude biological samples [2]. In this work, we demonstrate a self-contained, magnetically-actuated droplet cartridge for bisulfite treatment of genomic DNA with enhanced performance compared to the tube-based process.
我们报告了一个集成的数字微流控平台甲基化生物样品。表观遗传学研究的最新进展强调了DNA甲基化在癌症生物学中的重要性。ADAMTS1和BNC1基因启动子区域的表观遗传特征已被证明与胰腺癌的早期发生有很强的相关性[1]。大多数研究甲基化的分析技术需要通过亚硫酸盐转化对样品DNA进行预处理。然而,使用传统的管和移液器重复添加和移除液体试剂对微升规模的试剂来说是一个技术障碍,并且存在污染和人为错误的高风险。此前,我们报道了一种用于从粗生物样品中提取和扩增DNA的磁驱动平台[2]。在这项工作中,我们展示了一种独立的、磁驱动的亚硫酸氢盐处理基因组DNA的液滴盒,与基于管的工艺相比,它具有更高的性能。
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引用次数: 0
Combination of temperature cycled and gate bias cycled operation to enhance the selectivity of SiC-FET gas sensors 结合温度循环和栅极偏置循环工作,提高SiC-FET气体传感器的选择性
C. Bur, M. Bastuck, A. Schutze, A. Lloyd Spetz, M. Andersson
In this paper temperature modulation and gate bias modulation of a gas sensitive field effect transistor are combined in order to increase the selectivity. Data evaluation was performed using multivariate statistics, here by Linear Discriminant Analysis. It was found that both temperature cycling and gate bias cycling are suitable for quantification of different concentrations of carbon monoxide. However, combination of both approaches enhances the quality of the separation. In addition, the presented method allows discrimination of carbon monoxide, nitrogen dioxide and ammonia independent of the gas concentration.
本文将气敏场效应晶体管的温度调制与栅极偏置调制相结合,以提高选择性。数据评估采用多元统计进行,这里采用线性判别分析。结果表明,温度循环法和栅极偏置循环法均适用于不同浓度一氧化碳的定量。然而,两种方法的结合提高了分离的质量。此外,提出的方法可以区分一氧化碳,二氧化氮和氨独立的气体浓度。
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引用次数: 3
Microsystems and functional assays for mechanobiology 机械生物学的微系统和功能分析
B. Pruitt, J. Doll, S. Park, N. Harjee, B. Petzold
Microsystems and cantilever-based assays enable a wealth of custom measurements and analysis systems matched to the scale of cell biomechanics and mechanotransduction assays. Novel force and displacement sensors and actuators have been applied to study mechanisms of cell adhesion and the development and response of pluripotent cells in varied mechanical environments, to assay the function of hear cells, and to probe the mechanics and biology of the sense of touch in C. elegans or the mechanosensation of hair cells. In the Stanford Microsystems Lab, we design and fabricate many custom tools and sensors to probe cellular dynamics. We are interested in the reliable manufacture and operation of these micromachined devices in harsh biological environments as well as calibration approaches for precise measurements of nanoscale mechanical behaviors. We use these tools to address questions in the areas of physiology, biology, stem cells, neuroscience and cardiology with an eye toward quantitative and fundamental biophysics. In particular, microcantilevers have enabled measurements in a variety of applications over the past decades, ranging from scanning probe microscopy to visualize topography or characterize materials properties, to chemical sensing. Piezoresistive cantilever sensing offers particular advantages for system integration, scaling, and simplicity of transduction.
微系统和基于悬臂的分析使大量的定制测量和分析系统与细胞生物力学和机械转导分析的规模相匹配。新型的力和位移传感器和致动器已被应用于研究细胞粘附机制和多能细胞在不同机械环境下的发育和反应,分析听觉细胞的功能,探索秀丽隐杆线虫触觉或毛细胞机械感觉的力学和生物学。在斯坦福微系统实验室,我们设计和制造了许多定制工具和传感器来探测细胞动力学。我们感兴趣的是在恶劣的生物环境中可靠地制造和操作这些微机械设备,以及精确测量纳米级机械行为的校准方法。我们使用这些工具来解决生理学,生物学,干细胞,神经科学和心脏病学领域的问题,并着眼于定量和基础生物物理学。特别是,在过去的几十年里,微悬臂梁已经在各种应用中实现了测量,从扫描探针显微镜到可视化地形或表征材料特性,再到化学传感。压阻式悬臂传感为系统集成、缩放和简单的转导提供了特殊的优势。
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引用次数: 0
Autonomous microfluidic control by mixed potentials generated on composite metal electrodes 复合金属电极上产生混合电位的自主微流控
K. Kojima, M. Yokokawa, H. Suzuki
A new concept and devices for autonomous switching of valves and pumps in microfabricated flow channels are proposed. An electrowetting-based valve consisting of gold and zinc opened when an electrolyte solution reached the zinc part in a controlling flow channel and a mixed potential was applied to the gold valve electrode. Clock circuits that open the valves at predetermined times and logic circuits have been realized. A bubble micropump that moves a solution forward and backward was also constructed with three flow channels in which platinum black, zinc, and silver electrodes connected with each other. The technique was also used to create a device that ejects a solution in response to the concentration of creatinine and urea.
提出了一种微加工流道中阀门和泵自主开关的新概念和装置。当电解质溶液在控制流道中到达锌部分并在金阀电极上施加混合电位时,由金和锌组成的电润湿阀打开。实现了在预定时间开启阀门的时钟电路和逻辑电路。一个可以前后移动溶液的气泡微泵也由三个流动通道组成,其中铂黑、锌和银电极相互连接。该技术还被用于制造一种装置,该装置根据肌酐和尿素的浓度喷射出溶液。
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引用次数: 0
A microfluidic system with volume sensor and dielectric glucose sensor for continuous glucose monitoring 一种具有体积传感器和介质葡萄糖传感器的微流体系统,用于连续血糖监测
D. Li, H. Yu, X. Huang, B. Song, Y. Jia, Y. Ji, N. Li, J. Chen, K. Xu, Q. Lin
We present a microfluidic system for interstitial fluid (ISF) transdermal extraction, ISF volume measurement, and dielectric glucose sensing towards the application of continuous glucose monitoring. This device significantly incorporates a MEMS volume sensor, which measures extracted ISF volume via conductance monitoring, and integrates a MEMS dielectric glucose sensor, which specifically measures glucose concentrations in the transdermally extracted ISF via affinity binding. The volume sensor and the glucose sensor in the microfluidic system are characterized. The feasibility of the single-chip device for automated, accurate, and continuous monitoring of subcutaneous ISF glucose concentrations is verified.
我们提出了一种用于间质液(ISF)透皮提取,ISF体积测量和介质葡萄糖传感的微流体系统,用于连续葡萄糖监测。该设备集成了一个MEMS体积传感器,通过电导监测测量提取的ISF体积,并集成了一个MEMS介质葡萄糖传感器,通过亲和结合专门测量透皮提取的ISF中的葡萄糖浓度。对微流控系统中的体积传感器和葡萄糖传感器进行了表征。验证了该单芯片装置用于自动、准确和连续监测皮下ISF葡萄糖浓度的可行性。
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引用次数: 3
In vitro tumor model for evaluating the lung cancer induced tumor angiogenesis 评价肺癌诱导肿瘤血管生成的体外肿瘤模型
Tse-shao Chen, Kuo-Wei Chang, S. Sivashankar, Kang-Yun Lee, Cheng-Hsien Liu
Angiogenesis is not only physiological changes necessary for some process, but also some scientists have found it plays a role in development of cancer. When the tumor is newly formed, the cancer cell itself or the surrounding connective tissue secretes many proteins to promote angiogenesis. These substances will activate endothelial cells, for example: (a) decomposition of the tumor surrounding connective tissue damage; (b) endothelial cell proliferation; (c) the place of endothelial cells to promote the secretion of angiogenic substance transportation; (d) endothelial cells is reassembled into new blood vessels. We try to mimic the phenomenon of the angiogenic by chip.
血管生成不仅是某些生理过程所必需的生理变化,而且一些科学家已经发现它在癌症的发生发展中起着重要作用。当肿瘤刚形成时,癌细胞本身或周围的结缔组织分泌许多蛋白质来促进血管生成。这些物质会激活内皮细胞,例如:(a)分解肿瘤周围的结缔组织损伤;(b)内皮细胞增殖;(c)内皮细胞的分泌处促进血管生成物质的运输;(d)内皮细胞重新组装成新的血管。我们尝试用芯片模拟血管生成的现象。
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引用次数: 0
Functionalized carbon nanotubes for the discrimination of volatile organic compounds 功能化碳纳米管在挥发性有机物鉴别中的应用
I. Hafaiedh, W. El Euch, P. Clément, A. Abdelghani, E. Llobet
Oxygen plasma functionalized and decorated with iron oxide nanoparticle multiwall carbon nanotubes (MWCNTs) are used for detecting volatile organic compounds at room temperature. Sensors as the ones presented here, which can respond to aromatic VOCs and fully recover their baseline at room temperature, pave the way for a new generation of affordable, mass produced portable/ wearable detectors with important applications in continuous environmental monitoring or workplace protection.
多壁碳纳米管(MWCNTs)是一种在室温下检测挥发性有机化合物的材料。这里展示的传感器可以响应芳香族挥发性有机化合物,并在室温下完全恢复其基线,为新一代价格合理,批量生产的便携式/可穿戴探测器铺平了道路,在连续环境监测或工作场所保护中具有重要应用。
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引用次数: 1
Improvement of hot switching lifetime in MEMS DC switches using a drain voltage-sustaining capacitor 利用漏极电压维持电容改善MEMS直流开关的热开关寿命
Yong-Ha Song, Min-Woo Kim, Sangsoo Ahn, Jun‐Bo Yoon
Reliability in hot switching operations of MEMS DC switches is enhanced by introducing a drain voltage-sustaining capacitor, which leads to electric field-less or arc-less switching operations. The role of the capacitor is to eliminate the electric potential difference between the suspended beam and the drain electrode at the switch “opening” instant. In the case of a pure Au-Au contact, we have experimentally demonstrated that the lifetime of conventional MEMS switches with the suggested method was improved dramatically, by an order of magnitude, in the relatively high electric-field hot switching condition. We clearly observed material transfer at failure in the device without the capacitor, whereas stiction was the main failure mechanism in the device with the capacitor. Owing to the simplicity of the proposed method, it can be adopted in any type of MEMS DC switches in order to greatly enhance the switch lifetime.
通过引入漏极电压维持电容器来提高MEMS直流开关热开关操作的可靠性,从而实现无电场或无弧开关操作。电容器的作用是在开关“打开”的瞬间消除悬浮梁和漏极之间的电位差。在纯Au-Au触点的情况下,我们通过实验证明,在相对高电场的热开关条件下,使用该方法的传统MEMS开关的寿命显着提高了一个数量级。我们清楚地观察到,在没有电容器的设备中,材料在失效时转移,而在有电容器的设备中,粘滞是主要的失效机制。由于该方法简单,可用于任何类型的MEMS直流开关,从而大大提高开关寿命。
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引用次数: 5
Multidimensional metamaterial fluid sensor 多维超材料流体传感器
R. Lucklum, M. Zubtsov, A. Oseev, A. Omar, A. Martínez
We report for the first time on a fluid sensor platform applying simultaneously confined electromagnetic and acoustic waves. Confinement is realized in a so-called phoxonic crystal, a specific version of a metamaterial. Metamaterials are engineered to achieve desired effects by incorporating structural elements of (sub-)wavelength sizes. The sensor platform is a solid plate featuring an array of holes arranged in a square lattice. A defect introduces local resonances and the resonance frequency has been found to depend on refractive index or permittivity and mass attached to the defect.
我们首次报道了同时应用受限电磁波和声波的流体传感器平台。限制是在所谓的光子晶体中实现的,光子晶体是一种特殊的超材料。超材料是通过结合(亚)波长大小的结构元素来达到预期效果的。传感器平台是一个实心板,其特征是排列在方形晶格中的孔阵列。缺陷引入局部共振,并且发现共振频率依赖于附在缺陷上的折射率或介电常数和质量。
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引用次数: 0
期刊
2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII)
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