[Combined immunological and cytochemical identification of nucleated cells in normal human peripheral blood].

Abstract

Nucleated cells obtained from normal human peripheral blood on a layer of Ficoll Isopaque are identified according to the combination of various assays: phagocytosis, endogenous peroxidase, naphtol AS-D esterase, immunofluorescence (IF) performed at 4 degrees C and after incubation at 37 degrees C. The Ig bearing lymphocytes are evaluated with IF, while errors due to other nucleated cells may be evaluated by phagocytic and enzymatic capacities. As the presence of immunoglobulins (Ig) on the cell surface doses not prove its B lymphocytic nature, both immunofluorescence (IF) and endogenous peroxidase are usefully performed together. Exposure of the cells to 37 degrees C during half an hour may enable us to avoid to consider monocytes and lymphocytes with cell bound Ig. Thus con accurately be evaluated the percentages of lymphocytic populations in practice of immunological tests.