Quantitative detection of Escherichia coli and measurement of urinary tract infection diagnosis possibility by use of a portable, handheld sensor

Abstract

Electrochemical nitrite sensor was used to quantitatively detect the nitrite concentration in urine and by building the approximate relationship between the nitrite concentration and number of E.coli bacterias, electrochemical nitrite sensor can count the numbers of Escherichia coli and do the Urinary Tract Infection (UTI) Diagnosis. Electrochemical nitrite sensor was assembled and calibrated, the artificial urine sample was detected; the feasibility of electrochemical nitrite sensor including the errors effect had been checked and proved at around -5.1~2.3%; the possibility to detect artificial UTI urine sample out is around 95.5%; the approximate relationship between the number of E.coli and electrode potential had been built as E=228.3193-3.78225×Ln (N+2.29101e6), thereby building the relationship between UTI possibilities and the measurement. Finally, the conception and design of electrochemical sensor array had been made, thus to measure different biomarkers for the maximum possibilities of UTI and can show the data of the possibility of UTI directly on the screen. Furthermore, it can easily be used and transported for the home-users or patients in hospitals.