Quantitative Detection of Aflatoxin M1, Ochratoxin and Zearalenone in Fresh Raw Milk o f Cow, Buffalo, Sheep and Goat by UPLC XEVO - TQ i n Dakahlia Governorate, Egypt

Abstract

Animals’ derived food as milk is highly nutritive food for human and substitute mother’s milk for children especially after 6 months. To better produce and maintain sa fe milk and milk product, Ultra Performance Liquid Chromatography XEVO - TQ (UPLC) used to monitor mycotoxins as aflatoxin, ochratoxin and zearalenone levels in fresh raw milk of dairy domestic animals. AFL - M1 was detected in all samples in cow, buffalo, she ep and goat milk with different concentration ranged from high, medium and very low with mean 906, 811.1, 1394.86 and 1183.68 ng/L respectively. According worldwide standard for aflatoxin M1 level in milk as EC and according US FDA Limit, all samples of raw milk exceed this limit up to 100ng /l while 50%, 80, 90 and 60% exceed US FDA Limit. Notably, the Egyptian raw milk is free from ochratoxin and zearalenone mycotoxin. Taken collectively , strict strategies should be taken to reduce level of aflatoxin in raw milk between producer and consumer and so reduce risk of adverse effect on health.