A microfluidic aptasensor integrated with nanodrop generator for MALDI-TOF MS analysis in clinical applications

2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII) Pub Date : 2013-06-16 DOI:10.1109/TRANSDUCERS.2013.6627324

J. Yang, J. Zhu, Q. Lin

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Abstract

This paper presents an aptamer-based microchip for sensitive and quantitative detection of biomolecules using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS). The device is capable of both isolating analyte from samples and dispensing purified samples onto a probe plate for a following MS analysis. Aptamers selectively captured and released target molecules for isocratic elution. An integrated nanodrop generator produced small sample spots (300 μm diameter), significantly minimizing spatial intensity variations. Repeated sample deposition on the same spot (50 times) induced on-spot analyte concentration improving the detection sensitivity. We also demonstrated that our integrated device coupled to MALDI MS analysis successfully performs the biomolecular quantification by calibrating relative intensities of analyte peak to its internal standard.

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集成纳米液滴发生器的MALDI-TOF质谱分析微流控传感器在临床中的应用

本文提出了一种基于适配体的微芯片，用于基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)对生物分子的敏感和定量检测。该设备能够从样品中分离分析物，并将纯化的样品分配到探针板上，以便进行后续的质谱分析。适配体选择性地捕获和释放目标分子进行等温洗脱。集成的纳米液滴发生器产生小的样品点(直径300 μm)，显著减少空间强度变化。在同一地点重复沉积样品(50次)可诱导现场分析物浓度，提高检测灵敏度。我们还证明，我们的集成设备耦合到MALDI质谱分析成功地执行生物分子定量，通过校准分析物峰相对于其内标的相对强度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII)

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