Differentiation of Bicep femoris and Semimembranosus muscles of smoked dry-cured ham by quality parameters

Nives Marušić Radovčić, Ana Mikulić, M. Turk, H. Medić
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Abstract

The aim of this work was to determine the effect of fat and protein oxidation on the colour and texture of Biceps femoris (BF) and Semimembranosus (SM) muscles of smoked dry-cured ham Dalmatinski pršut. Fat and protein oxidation was determined by spectrophotometer methods of which fat oxidation by 2-thiobarbituric acid reactive substances method (TBARS) and protein oxidation by 2,4-dinitrophenylhydrazine (DNPH) method. Colour parameters (L*a*b* values) and texture profile analysis (TPA test) were also analysed. There was no statistical significant difference (P>0.05) between BF and SM in fat and protein oxidation. SM is an external muscle and is more exposed to oxygen than internal muscle BF. As a result of that SM showed slightly higher TBARS values than BF. Slightly higher values of carbonyls in BF can be explained by the higher water content in this internal muscle and thus by the stronger proteolytic activity. BF had higher L*, a* and b* values than SM. Higher values of adhesive force, cohesiveness and stringiness and lower values of hardness, adhesiveness, gumminess, chewiness and fracture were found for BF than for SM.
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烟熏干腌火腿股骨二头肌和半膜肌的质量鉴别
这项工作的目的是确定脂肪和蛋白质氧化对烟熏干腌火腿Dalmatinski pršut的股二头肌(BF)和半膜肌(SM)肌肉的颜色和质地的影响。采用分光光度计法测定脂肪和蛋白质氧化,其中脂肪氧化用2-硫代巴比妥酸反应物质法(TBARS)测定,蛋白质氧化用2,4-二硝基苯肼法(DNPH)测定。颜色参数(L*a*b*值)和纹理轮廓分析(TPA测试)也进行了分析。在脂肪和蛋白质氧化方面,豆粕与豆粕差异无统计学意义(P>0.05)。SM是一种外部肌肉,比内部肌肉BF更容易暴露于氧气。结果表明,SM的TBARS值略高于BF。BF中羰基值略高,这可以解释为内部肌肉中含水量较高,从而具有更强的蛋白质水解活性。BF的L*、a*和b*值均高于SM。与SM相比,BF的粘结力、黏结性和黏度较高,硬度、黏结性、胶性、咀嚼性和断裂性较低。
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