Development, bioactive and sensory analysis of the honey-filled chocolate pralines infused with ground ivy (Glechoma hederacea L.) extract

Danijela Šeremet, Petra Subotić, K. Marković, Ana Mandura Jarić, Aleksandra Vojvodić Cebin, Nada Vahčić, D. Komes
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Abstract

Chocolate is consumed largely worldwide and it is known as one of the most craved foods. The market of chocolate products is growing steadily and is expected to continue to grow in the coming years. The aim of the present study was to formulate chocolate pralines with enriched sensory and bioactive attributes by incorporating different types of honey (false indigo, buckwheat, rapeseed, mandarin and sage) and ground ivy (Glechoma hederacea L.) extract as ingredients of the filler. The honey samples were subjected to physico-chemical analysis, sugar analysis using the HPLC-RID methodology, as well as to evaluation of antioxidant capacity. The bioactive and sensory properties and sugar composition of the formulated pralines were determined. Bioactive characterization included the determination of phenolic profile by HPLC-PAD methodology. Buckwheat honey showed the most pronounced antioxidant capacity with the value of 3.21 and 2.06 µmol Trolox/g dmb, determined by ABTS and DPPH assays, respectively, while the lowest values were measured for mandarin honey (0.41 and 0.32 µmol Trolox/g dmb). Specific bioactive compounds of cocoa: epicatechin, theobromine and caffeine, were detected in all formulated chocolate pralines, as well as the most predominant phenolic compounds of ground ivy: chlorogenic, caffeic, rosmarinic acid and rutin. Such enriched bioactive composition contributed to the pronounced antioxidant capacity of chocolate pralines. Finally, chocolate pralines prepared with mandarin honey were sensory evaluated as the sweetest and gained the highest scores for the overall acceptability.
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常春藤(Glechoma hederacea L.)提取物浸泡蜂蜜巧克力果仁糖的开发、生物活性和感官分析
巧克力在全世界的消费量很大,被称为最受欢迎的食品之一。巧克力产品市场正在稳步增长,预计未来几年还将继续增长。本研究的目的是通过添加不同类型的蜂蜜(假靛蓝、荞麦、油菜籽、柑橘和鼠尾草)和常春藤(Glechoma hederacea L.)提取物作为填充物成分,配制出具有丰富感官和生物活性属性的巧克力果仁糖。对蜂蜜样品进行了理化分析,采用 HPLC-RID 方法进行了糖分分析,并评估了抗氧化能力。测定了配制果仁糖的生物活性、感官特性和糖成分。生物活性特性包括采用 HPLC-PAD 方法测定酚类物质的概况。通过 ABTS 和 DPPH 法测定,荞麦蜂蜜的抗氧化能力最强,分别为 3.21 和 2.06 µmol Trolox/g dmb,而柑橘蜂蜜的抗氧化能力最低(0.41 和 0.32 µmol Trolox/g dmb)。在所有配制的果仁巧克力中都检测到了可可的特定生物活性化合物:表儿茶素、可可碱和咖啡因,以及常春藤中最主要的酚类化合物:绿原酸、咖啡酸、迷迭香酸和芦丁。这种丰富的生物活性成分有助于提高巧克力果仁糖的抗氧化能力。最后,用柑橘蜜制作的巧克力果仁糖在感官评价中是最甜的,在总体可接受性方面得分最高。
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