Bioethanol Production From Rice Husk Through Shcf And Sscf Processing Strategies

O. C., Ikhuoria E. U., Obibuzo J. U.
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Abstract

In many developing countries, there is great quest for achieving sustainable energy from the conversion of the huge biomass of organic wastes into useful biofuels such as bioethanol. Bioethanol is a renewable clean-liquid biofuel produced by fermentation of sugars or converted starch or cellulose from plant based feedstocks. It is conventionally produced from sugar and starch containing feedstocks. However, these feedstocks are unable to meet the global demand of bioethanol production due to their primary food value and legal pursuits against the legitimacy of their schemes. This study investigated and improved on the feasibility of producing bioethanol from rice husk agro-waste generated from rice production. It was first subjected to different physico-chemical pretreatments in order to optimize the hydrolysate sugar yield and identify the most effective process. It was further hydrolyzed by cellulase enzymes from Trichoderma ressei micro-organism isolated from the soil. Separate hydrolysis and co-fermentation (SHCF) and simultaneous saccharification and co-fermentation (SSCF) strategies/methods were adopted using both hydrometer and Pycnometer measurements. The fermentation results revealed that the maximum bioethanol yield through SHCF and SSCF strategies were 4.64 and 6.45 (% w/v dry biomass) respectively. SSCF strategy was more effective as it gave better bioethanol yield and was less time consuming. This study also shows that rice husk agro-waste of no or little commercial value can be utilized in the production of good quality bioethanol with implications for improved waste management, income and efficient energy generation.
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通过Shcf和Sscf加工策略从稻壳生产生物乙醇
在许多发展中国家,迫切需要将巨大的有机废物生物量转化为有用的生物燃料,如生物乙醇,从而获得可持续能源。生物乙醇是一种可再生的清洁液体生物燃料,由植物原料中的糖或转化淀粉或纤维素发酵而成。它通常是由含有原料的糖和淀粉生产的。然而,由于其主要的食品价值和对其计划合法性的法律追求,这些原料无法满足全球对生物乙醇生产的需求。本研究探讨并改进了利用水稻生产过程中产生的稻壳农业废弃物生产生物乙醇的可行性。首先对其进行了不同的理化预处理,以优化水解糖的产率,确定最有效的工艺。用从土壤中分离出来的雷塞木霉微生物的纤维素酶进一步对其进行水解。采用比重计和体积计测量分别采用分离水解和共发酵(SHCF)和同时糖化和共发酵(SSCF)策略/方法。发酵结果表明,采用湿法发酵和湿法发酵的最大生物乙醇产量分别为4.64和6.45 (% w/v干生物量)。SSCF策略具有较好的生物乙醇产量和较短的时间消耗等优点。这项研究还表明,没有或几乎没有商业价值的稻壳农业废弃物可以用于生产优质生物乙醇,这对改善废物管理、提高收入和有效地发电具有重要意义。
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