Raptor

Abstract

Robust Alignment and Projection Estimation for Tomographic Reconstruction (RAPTOR) is a free available software to align raw stacks obtained from electron microscopes for tomographic purposes. It is intended to automatically obtain a full-precision alignment comparable to the one obtained with extended manual intervention. Fiducial particles are needed in the image for the alignment. It uses a probabilistic framework to deal with very noisy images like in thick cryo EM datasets. More information can be find in [1]. The main idea is to automate alignment, so users don’t have to spend too much time selecting gold particles.