In-situ single cell manipulation via nanorobotic manipulation system inside E-SEM

Yajing Shen, M. Nakajima, M. Ahmad, S. Kojima, M. Homma, T. Fukuda
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引用次数: 15

Abstract

We performed in-situ single cell (W303) manipulation with force measurement inside the Environment-SEM. The mechanical end effector was fabricated from a commercial AFM cantilever by FIB etching. The end effector was assembled to the nanorobotic manipulation system which can realize controlling the position at the nanometer scale. The releasing, sliding and rolling of single cell on a substrate were achieved by controlling the humidity condition inside E-SEM. The influence of humidity to the single cell manipulation inside E-SEM was also discussed for the first time. To our knowledge, it is the first time to manipulate single cell with sliding and rolling movements inside E-SEM.
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利用E-SEM内的纳米机器人操作系统进行原位单细胞操作
我们进行了原位单细胞(W303)操作,并在环境- sem内进行了力测量。以商用AFM悬臂梁为材料,采用FIB刻蚀法制备了机械末端执行器。将末端执行器装配到纳米机器人操作系统中,实现纳米尺度的位置控制。通过控制电子扫描显微镜内部的湿度条件,实现了单晶片在衬底上的释放、滑动和滚动。本文还首次讨论了湿度对电镜内单细胞操作的影响。据我们所知,这是第一次在电子扫描电镜内操纵单细胞滑动和滚动运动。
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