R. Bulan, Firmanta Sebayang, Emma Zaidar Nasution, Putri Wulandari
{"title":"Uji Aktivitas Antimikroba dan Antioksidan dari Ekstrak Etanol Daun Keji Beling (Strobilanthes crispus BI)","authors":"R. Bulan, Firmanta Sebayang, Emma Zaidar Nasution, Putri Wulandari","doi":"10.32734/ST.V2I2.511","DOIUrl":null,"url":null,"abstract":"Uji aktivitas antimikroba dari ekstrak etanol daun keji beling (Strobilanthes crispus BI) telah dilakukan terhadap mikroba Candida albicans, Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae dengan metode difusi cakram. Uji aktivitas antioksidan ekstrak etanol daun keji beling dilakukan dengan metode DPPH. Daun keji beling yang telah halus, diekstraksi dengan metode maserasi menggunakan pelarut etanol 96% selama 2 hari, dan dipekatkan dengan rotary vacum evaporator. Uji aktivitas antimikroba dilakukan dengan variasi konsentrasi 100; 200; 300; 400; 500 mg/ml. Hasil skrining fitokimia ekstrak etanol daun keji beling menunjukkan hasil positif terhadap senyawa golongan alkaloid dan fenolik. Hasil penelitian menunjukkan ekstrak etanol daun keji beling efektif terhadap mikroba Candida albicans dan tidak efektif terhadap mikroba Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae. Ekstrak etanol daun kejibeling mampu menghambat pertumbuhan Candida albicans dengan diameter zona bening untuk konsentrasi 100; 200; 300; 400; 500 mg/ml masing-masing adalah 11,53; 13,96; 15,46; 16,80; dan 18,20 mm. Uji aktivitas antioksidan ekstrak etanol daun keji beling dengan metode DPPH menunjukkan nilai IC50 sebesar 155,2 ppm. \n \nThe antimicrobial and antioxidant activity evaluation of leaves ethanol extract of KejiBeling (Strobilanthescrispus BI) has been carried out. The leaves of KejiBeling was grounded into powder and extracted using ethanol 96% (v/v) for 2x24 h and concentrated with rotary vacum evaporator. The antimicrobial activity was performed against several microbes, i.e. Candida albicans, Microsporumgypseum, Bacillus cereus, andShigella dysentriaeusing disk diffusion method. The antimicrobial activity was performed using several concentrations of leaves extract from 100-500mg/mL.The phytochemical screening showed the presence of alkaloid and phenolic group intheleaves ethanol extract of KejiBeling. The leaves ethanol extract of KejiBeling was antimicrobial active against Candida albicans with inhibition zone diameter of 11,53; 13,96; 15,46; 16,80; and 18,20 mm for the concentration of 100; 200; 300; 400; 500 mg/ml, respectively. The antioxidant activity of leaves ethanol extract showed IC50 value of 155,2 ppm","PeriodicalId":117967,"journal":{"name":"Talenta Conference Series: Science and Technology (ST)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talenta Conference Series: Science and Technology (ST)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32734/ST.V2I2.511","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Uji aktivitas antimikroba dari ekstrak etanol daun keji beling (Strobilanthes crispus BI) telah dilakukan terhadap mikroba Candida albicans, Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae dengan metode difusi cakram. Uji aktivitas antioksidan ekstrak etanol daun keji beling dilakukan dengan metode DPPH. Daun keji beling yang telah halus, diekstraksi dengan metode maserasi menggunakan pelarut etanol 96% selama 2 hari, dan dipekatkan dengan rotary vacum evaporator. Uji aktivitas antimikroba dilakukan dengan variasi konsentrasi 100; 200; 300; 400; 500 mg/ml. Hasil skrining fitokimia ekstrak etanol daun keji beling menunjukkan hasil positif terhadap senyawa golongan alkaloid dan fenolik. Hasil penelitian menunjukkan ekstrak etanol daun keji beling efektif terhadap mikroba Candida albicans dan tidak efektif terhadap mikroba Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae. Ekstrak etanol daun kejibeling mampu menghambat pertumbuhan Candida albicans dengan diameter zona bening untuk konsentrasi 100; 200; 300; 400; 500 mg/ml masing-masing adalah 11,53; 13,96; 15,46; 16,80; dan 18,20 mm. Uji aktivitas antioksidan ekstrak etanol daun keji beling dengan metode DPPH menunjukkan nilai IC50 sebesar 155,2 ppm.
The antimicrobial and antioxidant activity evaluation of leaves ethanol extract of KejiBeling (Strobilanthescrispus BI) has been carried out. The leaves of KejiBeling was grounded into powder and extracted using ethanol 96% (v/v) for 2x24 h and concentrated with rotary vacum evaporator. The antimicrobial activity was performed against several microbes, i.e. Candida albicans, Microsporumgypseum, Bacillus cereus, andShigella dysentriaeusing disk diffusion method. The antimicrobial activity was performed using several concentrations of leaves extract from 100-500mg/mL.The phytochemical screening showed the presence of alkaloid and phenolic group intheleaves ethanol extract of KejiBeling. The leaves ethanol extract of KejiBeling was antimicrobial active against Candida albicans with inhibition zone diameter of 11,53; 13,96; 15,46; 16,80; and 18,20 mm for the concentration of 100; 200; 300; 400; 500 mg/ml, respectively. The antioxidant activity of leaves ethanol extract showed IC50 value of 155,2 ppm