Influence of the receptor apparatus of the mammary gland on the content of estrogens during the lactation process in rats

Abstract

Purpose: To study the mechanisms of reducing the concentration of estrogens in the mammary gland.Materials and methods. In the experiment, breast preparations obtained from lactating rats were used. Estradiol receptors were detected using autoradiographic study and biochemical method in the cytosolic fraction. The specificity of estradiol binding was determined as the difference between the total binding during incubation in a medium containing 10-9 M ³H-17 β estradiol and binding during incubation in a medium containing, in addition to the labeled hormone, a 100-fold excess of unlabeled estradiol. During the washing process, structures with significant affinity for the hormone were revealed.Results. As a result of the study, the reception of estradiol by the cells of the alveolar part of the mammary gland during lactation was established. In the alveoli of the mammary gland, there is an intense binding of labeled estradiol by glandular cells and leukocytes. At high values of specific binding of estradiol, intensely labeled cells are located in the form of a close cluster. An indicator of the membrane binding of estradiol is the numerous silver granules accumulating over the cell border.Conclusion. The study made it possible to analyze the reception of estradiol in hormone-dependent tissue (mammary gland). Leukocyte cells have estrogen receptors, which reduce the level of the hormone as a result of lactation. In the mammary gland, there is a mechanism for reducing the concentration of estrogens. The ability of leukocyte receptor binding allows us to speak about the role of milk and colostrum cells in reducing the level of estrogens, which is one of the important factors in reducing the development of pathological processes in breast tissues.