Genetics and breeding of animals最新文献

{"title":"Physiological features of the cardiomodynamics of Jersey cows","authors":"E. Stepura, T. Ippolitova","doi":"10.31043/2410-2733-2024-1-67-73","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-67-73","url":null,"abstract":"Purpose: to establish indicators of cardiohemodynamic values and analyze them in Jersey cows.Materials and methods. In our studies, to measure and further analyze indicators of central and peripheral hemodynamics, we used a hardware-software complex for non-invasive study of central hemodynamics using the method of volumetric compression oscillometry KAP TsGosm-“Globus” (oscillometric analyzer of blood circulation indicators).Results. In the course of the study, in Jersey cows, average numerical values of myocardial-hemodynamic parameters were obtained: different types of blood pressure, vascular characteristics and indicators of cardiac activity. During mathematical analysis, hemodynamic parameters established the breed characteristics of Jersey cows. In this regard, it is advisable to include the assessment of these parameters in the basic set of methods for diagnosing heart diseases in cattle. The study of the cardiovascular system is of great importance in veterinary treatment and prophylactic work.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"3 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141380433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA fragmentation in spermatozoa of mammals and methods of its investigation (review)","authors":"M. Shubina, E. Korochkina","doi":"10.31043/2410-2733-2024-1-74-80","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-74-80","url":null,"abstract":"Purpose: systematization and analysis of scientific information on DNA fragmentation of spermatozoa of farm animals and methods of its research.Not long ago, one of the factors that negatively affects the fertility of male producers was discovered - fragmentation of sperm DNA. Numerous studies have established that DNA fragmentation is the breaking of DNA strands into pieces. According to Agarval A. (2003), sperm DNA fragmentation is the most common disorder of sperm ultrastructure. According to Baumber J. et al. (2003) determination of sperm DNA fragmentation is currently one of the modern methods for assessing sperm quality. Numerous studies have generated a pool of data on factors causing DNA fragmentation in sperm. Thus, Baumber J. et al. (2003) in their studies indicates that the cause of DNA fragmentation may be problems with chromatin remodeling, apoptosis and oxidative processes in sperm. Research conducted by Aboneev V.V. with coauthor. (2021) found that in severe forms of pathozoospermia, the number of sperm with fragmented DNA is higher than in less severe disorders of spermatogenesis [24]. The appearance of abnormal and inactive sperm in the ejaculate is one of the indirect markers of an increase in the index of DNA fragmentation in sperm, which negatively affects fertilizing ability of sperm. In this regard, the study of sperm DNA fragmentation is an effective diagnostic method for determining fertility. Considering some pathophysiological aspects leading to DNA fragmentation, determining the index of this process a priori cannot be routine. Currently, such high-precision methods as TTUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) — marking the ends of a broken molecule, SSCSA (Sperm Chromatin Structure Assay) — study of sperm chromatin dispersion, Comet (Single-cell gel electrophoresis assay, Cometassay) — capable of identifying breaks in single cells, SCD (Sperm Chromatin Dis) — are actively used. persiontest ) — chromatin dispersion test, etc.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"50 s248","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141377396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of new feed additives on the biochemical indicators of the blood of the Simmental breed in the conditions of Yakutia","authors":"N. Alekseeva, P. Borisova, N. Nikolaeva, S. Petrova, T. Rumyantseva","doi":"10.31043/2410-2733-2024-1-47-53","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-47-53","url":null,"abstract":"Purpose: to study the influence of new feed additives on the biochemical indicators of blood and the reproductive ability of the Simmental breed cows in Yakutia.Materials and methods. Studies were conducted on the basis of Khorobut LLC Megino-Kangalassky District of the Republic of Sakha (Yakutia) in the Earel dairy reproduct. In the summer, animals grazed on natural pastures in an agricultural economy, and in winter they were kept in a dairy reproduction on a leash. The duration of scientific and economic experience was 238 days. Animals were divided into 3 groups of 10 goals in each, given their age and live weight. The content of animals in groups was the same. Feeding was carried out twice a day. In the stall period, animals of all groups received a balanced diet in accordance with the detailed feeding system. All groups were contained in the same conditions, with the same diet. Bolus for dry cows was introduced using a special applicator. The diets for experimental groups were the same in basic nutrients and developed by A.P. Kalashnikov and colleagues. To assess the state of the animal body during the experience, a study of blood biochemistry was carried out, including the content of uniform elements and micro-macroelements.Results. The analysis of the data did not reveal statistically significant changes in the biochemical indicators of the blood during experiments. All blood parameters in all groups of animals remained within the limits of physiological norms both at the beginning and at the end of the experiment, without significant differences between groups. The results of the analysis of samples showed an increase in the level of total protein in the studied animals during experience. Although there were no statistically significant differences between the groups, there was only an increase in protein levels in the I-reserve group, while in the control and second groups this indicator remained stable throughout the experiment. Thus, the use of new feed additives for dry cows will adjust the metabolism and prevent productive disorders in their body.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141379693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of fertility indicators of breeding Ayrshire cows in the conditions of the European North of Russia","authors":"I. Komlyk, I. Lepesheva, A. Bolgov","doi":"10.31043/2410-2733-2024-1-39-46","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-39-46","url":null,"abstract":"The fertility of cows as the ability to produce offspring has a fundamental importance for optimizing the reproduction of the herd in dairy cattle breeding. With the increase in the value of milk yield in modern cows up to 8–10 thousand kg of milk and more, their reproductive abilities deteriorate.Objective: identification and characterization of associations of fertility indicators, their relationship, dependence on the level of milk yield in Ayrshire cows.Materials and methods. The research was held on cows of the Ayrshire breed of the breeding plant «Megrega» (Republic of Karelia). The average milk yield per cow is over 9000 kg of milk per year. Fertility indicators were taken into account for 5 years according to 3866 lactation data. The interrelations of these indicators and their associations with the value of milk yield were analyzed.Results. The age of the cows did not significantly affect on the fertility parameters, which were almost the same from the first to the fifth calving. An increase in the milk yield during the current lactation had a significant worsening effect on reproductive characteristics. The increase in milk yield from 5000 kg to 10000 kg and more was accompanied by an increase in their the indifference period (IP) by 38.4; the period of insemination (PI) – by 50.5; the service period (SP) – by 88.9 days; number of inseminations per conception – conception index (CI) – from 1.25 to 2.27; early embryonic death (EED) – from 3.1 to 13.3 % (P<0.001); fertilization from the first insemination – conception rate (CR) decreased from 78.1 to 33.6 %. The period from calving to the first insemination plays a special role among all fertility indicators. The best fertility rates were recorded in cows with an indifferent period of 100–120 days. A positive correlation was established between milk yield and IP (r = + 0.11–0.18, Р<0.001).Conclusions. The obtained results can be used in further research on the development of breeding indices, the selection of which can provide a high genetic trend in milk yield while maintaining and developing progressive fertility of dairy cows.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"116 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141376907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of immune mechanisms in liver tissues and blood plasma of rats against the background of loading with plant extracts and induced oxidative stress","authors":"O. Pavlova, O. Gulenko, D. Gromova, E. Kuzina","doi":"10.31043/2410-2733-2024-1-60-66","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-60-66","url":null,"abstract":"Pathologies of the hepatobiliary system occupy a significant share in the total structure of diseases. Morphological changes that develop in the liver during its toxic damage are based on cytolysis of hepatocytes. Metabolic disorders arising from exposure to tetrachloromethane may alter the production of anti-inflammatory and anti-inflammatory cytokines by cells of the immune system. The aim of our study was to investigate reparative processes and immune mechanisms in rat liver tissues against the background of loading with aqueous extracts of blackberry and common sawfly and induced oxidative stress.Materials and methods. 120 rats were used in the experiment. According to group affiliation animals received aqueous extracts of common and blackberry in a dose of 50 mg/100g of animal weight, 1.5 ml daily for 30 days, and animals of the control group received distilled water in the same regime. Starting from 30 days of the experiment, rats were injected with tetrachloromethane oil solution at a dose of 2 g/kg of animal weight in parallel with the administration of plant extracts for 6 days. On the 37th day of the experiment, blood was taken from the animals and then killed according to ethical standards. Histological preparations were made from liver tissues and the number of sinusoidal cells was counted. The content of cytokines in rat liver homogenates and blood plasma was determined by enzyme-linked immunosorbent assay using a Lazurite Automated ELISA System.Results. In case of oxidative stress in blood plasma there is an increased production of proinflammatory cytokine TNFα and suppression of IL-10 production, while the concentration of proinflammatory cytokines IL-6 and IL-18 does not change significantly. At the same time, in response to diffuse toxic liver damage and oxidative stress in liver tissues there is an increase in the concentration of cytokines IL- 1α, IL-18, TNFα, IL-10 and a decrease in the concentration of IL-6, IFNγ, TGF-β. Increased production of IL-1α and IL-18 in liver tissues seems to trigger local inflammation by increasing the levels of cytokines such as TNFα, IL-6. The increased concentration of anti-inflammatory cytokine IL-10 confirms the key role of this mediator in the regulation of immune response and its ability to suppress the secretion of pro-inflammatory cytokines TNFα and IL-6. Conclusion. The aqueous extracts of fir and blackberry modulate to different degrees the functional state of sinusoidal cells in the early periods of toxic exposure to tetrachloromethane, which contributes to the early resolution of the inflammatory process. Exposure of aqueous extracts of fir and blackberry to sinusoidal cells changes the production of regulatory factors, which compensates the speed of recovery processes after toxic exposure.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141380538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological adaptation features of yakut horses to extreme temperatures in the Arctic","authors":"I. Alferov, E. Sleptsov, I. Vinokurov, M. Savvinova, A. Nyukkanov, T. Rumyantseva","doi":"10.31043/2410-2733-2024-1-81-84","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-81-84","url":null,"abstract":"This article presents the results of the study of adaptive changes in hair cover in horses of Yakut breed of different populations, which contribute to the improvement of thermal insulation qualities in winter. In the study, it was found that horses of the Momsky population, in the period of severe cold weather, have the highest values of winter hair mass per 1 cm2 of skin (0.107 g) compared to the Amginsky population (0.070 g). In the first year of horses' life, hair growth is most intense and hair length reaches adult values by the age of 6 months. In Momsky horses, hair length is 37.5±0.5 mm and in Amginsky horses it is 46.0±0.68 mm. Long winter hair has adaptive value for foals of both populations. In summer, the average hair length in Amginsky foals is 5.54±0.04 mm, and in Momsky foals it is 6.0±0.8 mm. In the first winter, intensive hair growth is observed in Yakut horses, and the number of hairs per 1 cm2 of skin reaches the values of 1443±108 pcs/cm2 in Amginsky foals and 1812 ± 127 pcs/cm2 in Momsky foals. In summer, the thickest hairs with a diameter of about 53.4±1.40 μm were observed in Momsky foals and 53.1±1.0 μm in Amginsky foals. In winter, young foals from Mom ulus have absolute hair thickness values that are higher than those of young foals from Amga ulus. In winter, the thinnest hairs were observed in the Amginsky young stock, 55.3±1.2 µm, and in the Momsky young stock, 57.9±1.31 µm. In general, these results confirm the high thermal insulating ability of the hair coat in Yakut horses, which is an adaptation to the cold climate in Yakutsk horses.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"27 38","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141379922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of the protein profile of cows depending on the lactation period and method of housing","authors":"S. Kolomiets, L. Sosnina","doi":"10.31043/2410-2733-2024-1-54-59","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-54-59","url":null,"abstract":"Purpose: the study of the laws of protein metabolism of high -eating cows, depending on the period of lactation in the attached and unmistable ways of keeping animals in the conditions of the European North of Russia.Materials and methods. Biochemical studies were conducted with the aim of determining the protein profile of animals on the basis of the laboratory of biochemistry and physiology of animals using the equipment of the Center for Agricultural Research and Biotechnology Center for the Center for Agricultural Studies and Biotechnology, the Volnz RAS FSBBUN. The object of the study was the cows of the black-and-habitat of the Holstinized breed with a productivity of over 8500 kg in the household. When selecting groups of experimental animals, the physiological state, the stage of the sexual cycle and the method of content were taken into account. In total, 108 cows participated in the experience. Blood was taken from the tail vein in the morning in front of feeding. The conditions of feeding and content corresponded to the breed features of animals and ensured the development of high productivity indicators. To assess the condition of protein metabolism, the content was determined in the blood serum: total protein; Albuminov; alpha 1 globulin; Alpha 2 globulins; Beta Globulinov; Gamma Globulinov; protein index (the ratio of albumins to globulin). The material for the study was blood serum. In total, 108 samples were investigated.Results. The concentration of total protein in the blood serum of experienced animals is within fairly constant limits and the deviation is from 1 to 5 % lower than the established norm during the physiological cycle in the studied groups of animals. A connection has been established between the content of total protein and protein fractions in the serum of the studied animals and the method of detention. So the indicators of the total protein, the protein index and albumines with the tied method of content are higher than with the unmistable method of content during all periods of lactation. The maximum difference was detected in the period after the hotel (1-100 days of lactation) by the content of total protein and gamma globulin and amounted to 5 %. The smallest dependence of the indicators of the protein profile on the method of content was detected in animals during dryness. The level of total protein amounted to 8.0 and 7.99 g%, the content of albumins 3.07 and 3.04 g%, the protein index 0.6 and 0.58, with a binding and unmistable method of detention, respectively.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"136 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141376767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene polymorphism of the family of transcription factors and their influence on the milk productivity of the first heifers","authors":"I. Tkachenko, K. Fairushina, A. Kleshcheva","doi":"10.31043/2410-2733-2024-1-18-23","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-18-23","url":null,"abstract":"Purpose: this study investigated the polymorphism of transcription activators genes and their effects on milk production traits.Materials and methods. The first time in the Ural region, studies were carried out to determine the polymorphism of cattle in the STAT1 and STAT5A genes and the study of their associations with dairy productivity. Allele polymorphism was determined by the КASP method – Кompetitive Allele Specific PCR.Results. 92 cows were genotyped for the STAT1 gene locus, three genovariants were identified – STAT1СС (69,5 %), STAT1СТ (27,2 %) и STAT1ТТ (3,3 %). In the first lactation, the productivity of cows with the STAT1СС genotype was: milk yield 8379 kg, milk fat content 4.18 %, milk protein content 3.23 %. The individuals with STAT1СТ genotype had milk yield 8045 kg, MF 4.17 %, MP 3.23 %. And the individuals with genotype STAT1ТТ had milk yield 7855 kg, MF 4.16 %, MP 3.18 %. The advantage of cows with the STAT1CC genotype was 334-524 kg of milk compared to analogues from other groups. The genotypes of 90 cows were determined by the locus of the STAT5A gene. The STAT5ACT genotype is widespread among the studied livestock (51.1 %), the STAT5AСС genotype occurs with a frequency of 25.6 %, STAT5ATT genotype – with a frequency of 23.3 %. The productivity of cows with the STAT5ACC genotype was: milk yield of 8376 kg of milk, fat content of 4.19 %, protein content of 3.21%. The milk yield of the first heifers in the STAT5ATT and STAT5ACT groups is lower by 83–142 kg, the fat content is lower by 0.02–0.03 %. According to the results of the study, the STAT1 gene has an advantage for use as markers of high dairy productivity of Holstein cattle.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"30 2‐3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141383071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleotide diversity of the fragment of cytocrhome oxidase gene subunit 1 of mitochondrial DNA in (Oncorhynchus Nerka) populations","authors":"S. Pustovoit","doi":"10.31043/2410-2733-2024-1-24-30","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-24-30","url":null,"abstract":"Purpose: to analyze the nucleotide diversity of the cytochrome oxidase gene fragment of the 1 mt DNA subunit in the Asian and American populations of sockeye salmon.Materials and methods. The material for analysis is nucleotide sequences obtained by the author for the sockeye salmon population of the Ola River, and for a number of Asian and American sockeye salmon populations, located in the Gene Bank (www.ncbi.nlm.nih.gov). 139 sequences were analyzed, the unifies number of nucleotide was 640. The statistical parameters are as follows: ps – the proportion of polymorphic sites, θ - the proportion of polymorphic sites taking into account the number of sites (i) ps /a1 = 1 1i = 1 1 + ½ + 1/3 + … + 1/i, πT – nucleotide diversity, πs – average nucleotide diversity within a population, δST – interpopulation nucleotide diversity. NST – coefficient of nucleotide differentiation, NST = δST / πT. The dendrogram is built using the values of the so-called p-distances, using the UPGMA clustering method.Results. The nucleotide diversity of sockeye salmon from the Ola River (πT = 0,00505 (s=0,000080)) turned out to be slightly higher than in American populations (πT = 0,00403) (s=0,000002)). The coefficient of nucleotide differentiation NST and the number of polymorphic sites in Asian populations are higher than in American populations. Due to this, a higher level of nucleotide diversity was found in Asian sockeye salmon populations (πT = 0,00504 (s=0,000004)) than in North American ones. The nucleotide sequences of all individuals are divided into 2 groups. Individuals of these groups do not have territorial attachment, i.e. noted in both Asian and American populations. It is most likely that these groups formed as a result of Late Pleistocene climate fluctuations and the associated presence of two refugia on the Asian coast – the rivers of the northern coast of the Sea of Okhotsk and the rivers of the Kamchatka Peninsula, the second salmon, it is also assumed that two refugia will exist during the period of climate cooling, one in the area of the Alaska Peninsula, the second in the area of Vancouver Island. Almost all large populations of sockeye salmon are influenced by fishing and, in many cases, artificial farming. To reliably distinguish hatchery fish from sockeye salmon  produced in natural spawning grounds, the characteristic nucleotide sequence of the mtDNA cytochrome oxidase gene can be used.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"59 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141382994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Search for associations of single-nucleotide substitutions of the GOS2 gene with signs of body weight and fat deposition in broilers","authors":"O. Barkova, A. Krutikova, T. Larkina","doi":"10.31043/2410-2733-2024-1-31-38","DOIUrl":"https://doi.org/10.31043/2410-2733-2024-1-31-38","url":null,"abstract":"Purpose: Search for mononucleotide polymorphisms of the candidate gene G0S2 involved in lipid metabolism and study their relationship with abdominal fat content and body weight in broiler chickens.Materials and methods. The experiments used 150 broilers of the Isa Hubbard F-15 cross at the age of 35 days from a private farm (2015). For sequencing, we used chickens of the Pushkin breed (23 individuals) aged 475 days, kept on the basis of the bioresource collection of the VNIIGRZh “Genetic collection of rare endangered breeds of chickens” (Pushkin, St. Petersburg). Identification of mononucleotide polymorphisms of the G0S2 gene was carried out by sequencing regions containing coding and regulatory sequences. Genotyping using the amplification method for testing test systems was carried out on a Thermal Cycler T100 device (Bio-Rad, USA).Results. Using sequencing of the regulatory region of the G0S2 gene, two mononucleotide polymorphisms were identified: rs29005090 (A/G) and rs317858728 (A/G), as well as a significant influence of all three genotypes (AG, AA, GG) of the rs29005090 substitution on the studied traits. Mononucleotide substitution rs29005090 is classified as major, since the effect of allele substitution is more than 0.6–1.5 sigma. The GG genotype rs29005090 can be recommended for marker-assisted selection to increase poultry weight gain and reduce abdominal fat of chicken carcasses in meat poultry farming. When conducting an analysis of variance of data to calculate the effect of substitution of alleles of the mononucleotide substitution marker rs317858728 (A/G) with the traits bird weight and abdominal fat weight of broilers of the Isa Hubbard F-15 cross, a significant difference was revealed between genotypes AA-GG for the traits bird weight and for the sign abdominal fat mass. The highest indicators for body weight and abdominal fat mass were observed in chickens with the GG genotype. This mononucleotide substitution is considered major in relation to the trait “abdominal fat”, since the standard deviation is more than one sigmа.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"24 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141382799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}