Suitability of antidigoxin antiserum for digoxin in plant extracts.

Lloydia Pub Date : 1977-05-01
S L Nickel, E J Staba
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Abstract

Antiserum specificity for digoxin was demonstrated by means of three tests: cross-reactivities test, dilutions test and thin-layer chromatography test. The cross-reactivities test examined quantitatively the cross-reactivities with specific compounds and provided an explanation for the basis of the dilutions test. The dilutions test was used to demonstrate the suitability or unsuitability of the antiserum for use in the radioimmunoassay of digoxin in a crude biological sample. The tlc test examined for antiserum cross-reactivity with digoxin and related compounds in a crude extract.

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地高辛抗血清对地高辛植物提取物的适用性。
通过交叉反应试验、稀释试验和薄层色谱试验验证了地高辛的抗血清特异性。交叉反应性试验定量地考察了与特定化合物的交叉反应性,为稀释试验的基础提供了解释。稀释试验是用来证明抗血清适合或不适合用于地高辛在粗生物样品的放射免疫测定。薄层色谱法检测了与地高辛及相关化合物在粗提取物中的抗血清交叉反应性。
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