Surface Plasmon Resonance based on phase detection technique for glycerin analysis

Abstract

Surface Plasmon Resonance (SPR) has become a popular method for the analysis of molecular interaction. There are many different approaches for the detection of SPR, however, the Phase detection method is quite sensitive in that it shows a much sharper change under SPR compared with other alternatives such as intensity and angle interrogation techniques. The SPR phase sensor can detect very low concentration levels of Glycerin that is not possible with intensity and angle based detection methods. Experimental results obtained from Glycerin-Water mixture show that SPR Phase interferometer with continuous phase change allows the detection of very low levels of Glycerin concentrations. Such an improvement in SPR Phase sensor can make it a more suitable option for biomedical applications.