Preservation of Peripheral Blood Stem Cells (CD34+/CD38-) for Bone Marrow Transplantation in Thai Lymphoma Patients

Abstract

Abstract Hematopoietic stem cell transplantation (HSCT) is one method of lymphoma therapy. Peripheral blood stem cell (PBSC) separation, preservation, cell viability, and cell function before transplantation are important factors in the success rate of stem cell transplantation. This study aims to separate and estimate the efficiency of deep-freezing preservation in autologous PBSCs (CD34+/CD38-) from lymphoma patients undergoing stem cell transplantation. PBSCs were separated and collected by leukapheresis before being cryopreserved and stored in liquid nitrogen. The number of CD34+/CD45dim cells was investigated and compared with the subpopulation of CD34+/CD38- cells using conventional trypan blue exclusion method and 7-AAD before and after cryopreservation. Colony-forming units (CFUs) were determined to indirectly assess the viability and potency of the PBSCs. The result showed that CD34+/CD38– cells constituted 35.56% of total CD34+ cells and 0.05% of total nucleated cells (TNCs). After thawing, the number of CD34+/CD38- cells did not demonstrate significant differences compared with pre-storage. The percentage of CFU recovery was 94.74%. In this study, the storage process of deep-freezing cryopreservation demonstrated high-quality recovery of CD34+/CD38- cells from PBSCs for autologous hematopoietic stem cell transplantation in lymphoma patients. This result showed novel data about the preservation of CD34+/CD38- cells. Keywords: Lymphoma, Autologous peripheral blood stem cell, Blood cryopreservation, Bone marrow transplantation