Increased activity of the pro/antioxidant system of potato microplants by rhizospheric bacterias in aeroponics conditions

O. Tkachenko, N. Evseeva, Kristina Yurievna Kargapolova, Alena Yurievna Denisova, N. Pozdnyakova, A. A. Kulikov, G. Burygin
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Abstract

The improvement of plants in in vitro culture is an obligatory stage of potato seed production. Plant-growth-promoting rhizobacteria contribute to an increase in plant resistance to stress factors, including at the stage of adaptation of microplants to ex vitro conditions. The purpose of this investigation was to study the mechanisms of antioxidant protection of potato microclones during inoculation with rhizobacteria in the process of their adaptation to ex vitro conditions in an aeroponic installation. In experiments, microplants of potato Nevsky and Condor cultivars inoculated in vitro with rhizobacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7 were used. In the process of adaptation in aeroponics conditions, microplants were analyzed for morphometric and biochemical parameters for 21 days. It was found that the bacterization of microplants contributed to the regulation of the pro/antioxidant system, leading to a decrease in oxidative stress and better and faster survival of plants ex vitro.
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气培条件下根际细菌对马铃薯微植株促/抗氧化系统活性的影响
离体培养植株的改良是马铃薯制种的必经阶段。促进植物生长的根瘤菌有助于提高植物对胁迫因素的抗性,包括在微植物适应离体条件的阶段。本试验旨在研究马铃薯微无性系在体外气培环境适应过程中,接种根杆菌对其抗氧化保护的机制。本试验以马铃薯和秃鹰品种的微株为材料,分别接种baldanorum Azospirillum Sp245和cytisochrobactrum IPA7。在适应气培条件的过程中,对微植物进行了为期21 d的形态计量学和生化指标分析。结果表明,微植物的杀菌作用有助于调节促氧化/抗氧化系统,从而降低植物的氧化应激,提高植物的离体存活率。
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