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Identification of effective primers for varieties of winter rye Secale cereals L. 冬麦黑麦品种有效引物的鉴定。
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp46-50
V. Pechenkina, A. I. Vshivkova, Ilya Alekseevich Tarbeev, R. Vyatkina, Konstantin Arturovich Maron
Rye (Secale cereale L.) is important food crop in Russia. The study of the genetic diversity of crops is carried out using molecular markers. To identify effective primers, ISSR (Inter Simple Sequence Repeats), a method for determining DNA polymorphism, was used.The selection of ISSR primers was carried out according to the largest number of polymorphic DNA fragments, their clear amplification and reproducibility in repeated PCR. Of the 22 analyzed ISSR primers with the total DNA of S. cereale, 5 ISSR primers demonstrated high efficiency for the studied 5 winter rye varieties. For further molecular genetic analysis of S. cereale, the following four dinucleotide ISSR primers (M1 (AC)8CG, ISSR-5 (AG)8CA, CR-215 (CT)6TG, CR-218 (GA)6CC) and one trinucleotide ISSR primer X10 (AGC)6C. The identified ISSR primers will make it possible to determine the genetic
黑麦(Secale cereale L.)是俄罗斯重要的粮食作物。利用分子标记技术对作物遗传多样性进行研究。为了鉴定有效引物,采用了DNA多态性测定方法ISSR (Inter Simple Sequence Repeats)。ISSR引物选择依据多态性DNA片段数量最多、扩增清晰、重复PCR重现性好。在22条含小麦总DNA的ISSR引物中,有5条引物对5个冬黑麦品种的ISSR效率较高。为了进一步对谷类小麦进行分子遗传分析,我们选用了4条二核苷酸ISSR引物(M1 (AC)8CG、ISSR-5 (AG)8CA、CR-215 (CT)6TG、CR-218 (GA)6CC)和1条三核苷酸ISSR引物X10 (AGC)6C。鉴定出的ISSR引物将使其遗传鉴定成为可能
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引用次数: 0
Families of Brown Swiss cows in breeding farms of the Smolensk region 斯摩棱斯克地区养殖场的棕色瑞士奶牛家族
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp70-74
D. Leutina, M. E. Gontov, E. Prishchep, A. Gerasimova
Increasing the dairy productivity of cows is the main goal of breeding work in dairy cattle breeding. The main method of purebred cattle breeding is considered to be breeding by lines and families. In the herds of five padigree farms of the Smolensk region for the breeding of the Brown Swiss breed of cattle 160 main families with a total number of 2285 cows have been established. The number of families on farms ranged from 17 to 40 with the number of animals from 235 to 502, in each. The main indicators of dairy productivity of cows in individual families are determined, the most promising for further breeding work are identified. The best families were formed in the breeding reproducer of the SPC "Druzhba" with record indicators of dairy productivity of cows for the breed. So in the Nevesta's family 917 cows were received: Zlata 2259 (3 – 305 – 8953 -3.94% – 3.31%); Dona 313 (2 – 305 – 11184 – 3.76% – 3.64 %). The Index of genetic similarity between animals of individual families calculated using immunogenetic markers revealed significant differences in the hereditary characteristics of the compared groups.
提高奶牛的产奶量是奶牛育种工作的主要目标。纯种牛的主要饲养方法是按系、按家饲养。在斯摩棱斯克地区饲养瑞士棕色牛品种的五个血统农场的牛群中,已经建立了160个主要家庭,总数为2285头牛。农场上的家庭数量从17个到40个不等,每个家庭的牲畜数量从235只到502只不等。确定了个体奶牛产乳率的主要指标,确定了最有希望进行进一步育种工作的奶牛。在“德鲁日巴”的繁殖繁殖中形成了最佳的家族,该品种奶牛的产奶量指标达到了记录。因此,Nevesta家族收到了917头奶牛:Zlata 2259头(3 - 305 - 8953 -3.94% - 3.31%);Dona 313(2 - 305 - 11184 - 3.76% - 3.64%)。利用免疫遗传标记计算个体家系动物遗传相似性指数,结果显示各组遗传特征存在显著差异。
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引用次数: 0
Results of studies on the sealing of crop residues of leguminous crops with ploughshares 豆科作物秸秆用犁头封堵的研究结果
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp122-127
Vasily Mikhailovich Boikov, S. Startsev, A. Pavlov, E. S. Nesterov
The article presents the results of experimental studies of the sealing of crop residues and their distribution over the depth of the cultivated soil layer by known general-purpose ploughshares without ploughshares. The research was carried out in fields with agrophones of winter wheat, chickpeas and winter rapeseed. The plowing of dark chestnut soils located in the steppe soil-climatic zone of the Left-Bank district of the Saratov region was carried out by aggregates K-701+PNL-8-40, K-701+PSK-8 and T-150K+PLN-5-35. As a result of field experiments, it was found out that straw, pollen and stubble are embedded in the soil in accordance with established agrotechnical requirements, more than 80%. Crop and plant residues are located in the bulk in the deep layers of the cultivated soil. Part of the stubble in an inclined position is located in the places where the layers of adjacent passages of the plow hulls meet.   The design of the ploughshare-dump surface of the housing does not allow managing the upper organic-enriched soil layer.
本文介绍了用已知的通用犁头对作物残茬的密封及其在耕地土层深处分布的实验研究结果。在冬小麦、鹰嘴豆和冬油菜籽的田间进行了试验研究。采用K-701+PNL-8-40、K-701+PSK-8和T-150K+PLN-5-35对位于萨拉托夫州左岸草原土壤-气候带的黑栗子土进行耕作。通过田间试验发现,秸秆、花粉、残茬按既定农业技术要求埋在土壤中的比例达80%以上。农作物和植物的残余物大量地埋在耕地的深层中。倾斜位置的部分残茬位于犁壳相邻通道层相交的地方。房屋的犁头-排土场表面的设计不允许管理上层有机富土层。
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引用次数: 0
The effectiveness of various methods of protection against hemp flea and the use of dressing from root rot in the hemp cultivation 不同防蚤方法的效果及根腐剂在大麻栽培中的应用
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp51-57
I. I. Pluzhnikova, N. V. Kriushin, I. V. Bakulova
The paper analyzes the influence of protective measures, seed dressing and ground processing of non-narcotic hemp fields on the morphometric parameters of plants, damage by pests and diseases, and crop yield. The article focuses on the effect of dressing agents in various combinations on the growth processes of cannabis seeds in the early stages of development. In tests, laboratory and field, seed treatments with Benorade and Celeste Top played the greatest stimulating role. The best results in reducing plant damage by Psylloides attenuate (Koch) 66.7 and 60.0% with a duration of protection of up to four to five pairs of leaves were obtained with the use of Tabu + Albit preparations for seed treatment and Samurai Super insecticide for ground application. With the help of Celeste Top in combination with the studied fungicides, root rot was suppressed, while the prevalence of the disease was reduced by 24.4 and 24.8% compared with the absolute control. A high increase in the yield of stems was guaranteed by the treatment of plants with an insecticide and seeds with Celest Top preparations in combination with Albit and Benorad 2.19 and 2.10 t/ha. A significant increase in seed yield was recorded in the variants when seeds were disinfected with the developed compositions Bunker + Celeste Top, Taboo and Benorade + Celeste Top 0.29 and 0.24 t/ha with the involvement of an insecticide during ground cultivation of the crop.
分析了非麻醉大麻田的防护措施、种籽处理和地面处理对植物形态计量参数、病虫害危害和作物产量的影响。本文着重研究了不同组合敷料剂对大麻种子发育早期生长过程的影响。在试验、室内和田间试验中,贝诺拉德和赛莱斯特托普种子处理的刺激作用最大。种子处理使用Tabu + Albit制剂,地面施用Samurai Super杀虫剂,对衰减木虱(Psylloides atten衰减木虱(Koch))的防治效果为66.7%和60.0%,保护期可达4 ~ 5对叶片。与绝对对照相比,Celeste Top与所研究的杀菌剂联合施用可抑制根腐病,根腐病患病率分别降低24.4%和24.8%。用一种杀虫剂处理植株,用Celest Top制剂与Albit和Benorad(2.19和2.10吨/公顷)联合处理种子,保证茎部产量的高增长。在作物地面种植期间,使用已开发的组合物Bunker + Celeste Top、Taboo和Benorade + Celeste Top 0.29和0.24 t/公顷进行消毒,并使用杀虫剂,记录到这些变种的种子产量显著增加。
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引用次数: 0
Evaluation of treatment methods for cows with subclinical endometritis 奶牛亚临床子宫内膜炎治疗方法的评价
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp87-90
A. M. Semivolos, Valery Aleksandrovich Agoltsov, S. A. Semivolos, Larisa Pavlovna Padilo, Glafira Dmitrievna Skvortsova
The article presents the results of the spread of various forms of endometritis in cows. At the same time, the subclinical form of endometritis was most often recorded (22.48%). Intrauterine administration of Metricef allows achieving fruitful insemination of cows (82.35%). Positive results in restoring fertility in cows with subclinical endometritis can also be obtained after treatment with the anti-mastitis preparation dienomast. Fertilization of animals after its application was 76.47%.
本文介绍了各种形式的子宫内膜炎在奶牛中传播的结果。同时,亚临床形式的子宫内膜炎记录最多(22.48%)。宫内给药Metricef可使奶牛获得有效的授精(82.35%)。抗乳腺炎制剂dienomast对亚临床子宫内膜炎奶牛的生育能力恢复也有积极的效果。施用后动物受精率为76.47%。
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引用次数: 0
Optimal operating modes of the agricultural implement position control device in the unit 机组农机具位置控制装置的最佳工作方式
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp147-150
S. Semichev, R. Filippov
The article presents the results of a field experiment of a developed controlled attachment device (UNU-3) as part of an aggregate with an MTZ-1221 tractor and a mounted cultivator KON-2.8 and provides recommendations for its use. It was found that when using a controlled mounted device, the range of variations in the numerical values of the deviation of the cultivator axis from the line set by the navigation equipment at the speed of the unit from 1.39 m/s to 1.94 m/s ranged from 0.08 to 0.04, which is 2-4 times less than without using the device, respectively. The process of functioning of a controlled attachment is described. It is determined that this device will increase the positioning level of the aggregated gun by minimizing the transverse displacement.
本文介绍了一种开发的控制附着装置(UNU-3)在MTZ-1221拖拉机和KON-2.8悬挂耕耘机上作为集料的一部分进行现场试验的结果,并提出了使用建议。结果表明,在机组运行速度1.39 ~ 1.94 m/s范围内,使用控制挂载装置时,耕耘机轴线偏离导航装置设定直线的数值变化范围为0.08 ~ 0.04,分别比不使用该装置时减小了2 ~ 4倍。描述了受控依恋的功能过程。确定该装置将通过最小化横向位移来提高聚合枪的定位水平。
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引用次数: 0
Species composition and features of weed control on irrigated lands in the system of forest strips 林带灌溉地杂草控制的物种组成及特征
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp40-45
A. V. Panfilov, V. Popov, Olga Gennadievna Udalova, Natalia Evgenievna Kuryleva, E. G. Panfilova, Evgeny Nikolaevich Martynov
Under irrigation conditions, weeds reduce agricultural production. On irrigated lands in the conditions of the Volga region in the system of forest strips, weeds grow faster. The measures applied in the fight against weeds, depending on the species composition, are primarily an obstacle to their penetration into the fields and measures that are used in the fight against weeds in the irrigated field. In agricultural production, when irrigation is carried out in the system of forest strips, weed control must be carried out taking into account the species composition of weeds that determine the type of contamination and the economic threshold of harmfulness. Agrotechnical weed control measures have the maximum effect in the weed control system, primarily in combination with chemical protection of crops from weeds.
在灌溉条件下,杂草减少农业产量。在伏尔加河地区林带系统的灌溉土地上,杂草生长得更快。除草所采取的措施,取决于杂草的种类组成,主要是防止杂草渗入田地的障碍,以及在灌溉区除草所采取的措施。在农业生产中,在林带系统进行灌溉时,必须考虑到决定污染类型和危害经济阈值的杂草种类组成进行杂草控制。农业技术除草措施在除草系统中效果最大,主要是与作物的化学防护相结合。
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引用次数: 0
Fatty acid composition of beef and pork with different types of animal fattening 牛肉和猪肉的脂肪酸组成与不同类型动物的育肥有关
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp97-101
Pyotr Ivanovich Tishenkov, A. A. Vasiliev, Dmitry Vladimirovich Bykov
The article presents data on the chemical, fatty acid composition of beef and pork meat in various types of fattening. The nutritive value of beef obtained from grain and traditional cattle feeding, as well as concentrate-type pork was studied. Significant differences in the content of protein, fat, individual fatty acids in meat are shown: saturated, unsaturated and polyunsaturated. It has been established that the concentrate type of feeding cattle leads to a violation of scar digestion and metabolic processes in the animal body. Long-term, for 200 days, feeding a large amount of grain leads to a fivefold increase in the content of fat in beef, a decrease of 23.2% in the concentration of protein in muscle tissue compared to beef obtained according to traditional technology and 3.1% with concentrate-type pork of fattening, therefore, and quality.
本文介绍了不同育肥方式下牛肉和猪肉的化学成分和脂肪酸组成。研究了谷物牛肉、传统养牛牛肉和精料型猪肉的营养价值。肉类中蛋白质、脂肪和个别脂肪酸的含量有显著差异:饱和、不饱和和多不饱和。已经确定的是,浓缩型饲养牛会导致动物体内疤痕消化和代谢过程的破坏。长期饲养200天,大量饲喂谷物使牛肉脂肪含量提高了5倍,肌肉组织中蛋白质浓度比传统工艺生产的牛肉降低了23.2%,比精料型育肥猪肉降低了3.1%,从而提高了品质。
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引用次数: 0
Dairy productivity of Swiss cows of different origin 不同产地瑞士奶牛的产奶量
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp102-106
I. Tletseruk, N. Konik, V. V. Golembovsky, M. Ulimbashev
The article presents the results of a comparative assessment of the dairy productivity of Swiss cows of different origin. The superiority in milk yield of the first heifers of the brown Swiss breed of Austrian breeding in all months of lactation over the peers of other groups has been established. At the same time, the lactation curves of imported heifers and their hybrids with local Shvits indicate that the maximum levels of productivity were observed in earlier periods – for 2-3 months lactation, whereas in individuals of the local population – for 3-4 months. After the peak of the productivity of the experimental livestock, the Austrian and crossbred Swiss heifers, whose lactation curve was relatively equalized, were the best preserved at the achieved milk yield level, while the local Swiss heifers had a sharp decline. The highest level of milk yield was distinguished by the first heifers of imported breeding, which surpassed both half-blooded peers (by 686 kg, P>0,999) and individuals of the local population (by 1656 kg, P>0,999). Imported animals, as well as crossbreeds, exceeded the yield of milk fat by 37,8-62,7 kg (P>0,999) and milk protein by 31,8–54,1 kg (P>0,999) of the peers of the brown Swiss breed of the local population.
本文介绍了对不同产地的瑞士奶牛的产奶能力进行比较评估的结果。在哺乳期的所有月份里,奥地利繁殖的棕色瑞士品种的第一头小母牛的产奶量优于其他群体的同类,这一点已经确立。与此同时,进口小母牛及其与当地Shvits杂交的泌乳曲线表明,在较早时期(泌乳2-3个月)观察到最高生产力水平,而在当地种群个体中,最高生产力水平为3-4个月。在试验家畜生产能力达到峰值后,泌乳曲线相对均衡的奥系和杂交瑞士小母牛在达到产奶量水平上保持得最好,而瑞士本地小母牛则急剧下降。产奶量最高的是进口的第一头小母牛,其产奶量超过了同血统的同类(686公斤,P>0,999)和当地人口个体(1656公斤,P>0,999)。进口动物和杂交品种的乳脂和乳蛋白产量分别比当地人口的瑞士棕色品种高出37,8-62,7 kg (P>0,999)和31,8 - 54,1 kg (P>0,999)。
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引用次数: 0
Infestation of white mustard agrocenosis, depending on agricultural practices in the conditions of the forest-steppe of the Middle Volga region 在伏尔加河中部地区的森林草原条件下,白芥菜病的侵袭取决于农业实践
Pub Date : 2023-06-26 DOI: 10.28983/asj.y2023i6pp12-17
Vera Aleksandrovna Gushchina, A. S. Lykova, Alexander Sergeevich Korolev
To obtain environmentally friendly products, in the technology of cultivating agricultural crops, the basis of modern weed control should be agricultural practices. In this regard, the purpose of the research is to select the optimal seeding rate and sowing method for white mustard, which increase the self-regulation of the number of weeds in the agrocenosis to obtain the highest seed yield. The experiment was carried out in 2020–2022 on the meadow-chernozem soil of the experimental plot of the Federal State Budgetary Educational Institution of Higher Education Penza State Agrarian University. The weather conditions during the years of research were different, but they met the requirements for the growth and development of white mustard. The growing season of mustard in the first and third years passed under conditions of sufficient moisture at the optimum temperature regime (HTC 1.3 and 1.0, respectively). In the second year of research, drier conditions developed, when the hydrothermal coefficient was 0.87. On average, the minimum infestation of mustard crops before harvesting was traced with an ordinary sowing method of 36-51 pcs/m2 with a wet weight of weeds of 75.8-106.0 g/m2, while with a wide-row method their number was 43-62 pcs/m2 with a mass of 91.0-129.2 g/m2, where the feeding area increased not only for cultivated, but also for weeds. Moreover, the agrocenosis was the cleanest with the maximum seeding rate (3 million germinating seeds/ha) and mustard sowing with a row spacing of 15 cm, where the vegetative mass actively suppressed weeds. The highest infestation in terms of quantity and weight of the weed component was established at a rate of 1.5 million and mustard sowing with a row spacing of 30 cm. Plants with row sowing with a seeding rate of 2.0-2.5 million germinating seeds/ha were more competitive, while providing a maximum oilseed yield of 2.46-2.58 t/ha.
要获得环境友好型产品,在农作物栽培技术上,现代杂草防治的基础应该是农业实践。因此,本研究的目的是为白芥菜选择最优的播量和播种法,以增加油菜中杂草数量的自我调节,从而获得最高的种子产量。试验于2020-2022年在奔萨州立农业大学联邦国家预算高等教育机构试验区的草甸黑钙土上进行。研究期间的气候条件各不相同,但都符合白芥菜生长发育的要求。第一年和第三年的芥菜生长季节是在水分充足、温度适宜(HTC分别为1.3和1.0)的条件下度过的。在研究的第二年,水热系数为0.87,条件更加干燥。普通播种法收获前平均最小侵染量为36 ~ 51支/m2,杂草湿重为758 ~ 106.0 g/m2;宽行播种法收获前平均最小侵染量为43 ~ 62支/m2,杂草湿重为91.0 ~ 129.2 g/m2,不仅增加了栽培面积,而且增加了杂草的取食面积。其中,植瘤最干净,出苗率最高(300万萌发种子/ha),行距为15 cm的芥菜播种,营养团对杂草有积极抑制作用。以排播量150万株和行距30 cm的芥菜播种量和重量为最大侵染量。排播200万~ 250万萌发种子/公顷更具竞争力,油籽产量最高可达2.46 ~ 2.58 t/公顷。
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引用次数: 0
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The Agrarian Scientific Journal
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