Peroxidase isoform activity in spring wheat grain under the influence of growth regulators

Abstract

In laboratory experiments, the effect of various growth biological preparations on the activity of peroxidase isoforms in dormant seeds and during their germination was studied. In the experiment, at pH = 5.5, 7.0, 8.0, the activity of enzyme isoforms was determined using a phosphate-buffer system (1/15 M). The activity of isoforms of the peroxidase enzyme (by a method based on tyrosine peroxidation) was determined on dormant wheat grains (after ripening after harvesting) and germinated on the 3rd, 5th, and 7th days of germination. According to the research results, different effects of the biological preparations used (protective-stimulating complex, Hardy, Ferovit and Ferovit + Hardy mixtures) on the activity of peroxidase isoforms in spring wheat grain were noted, which depended on the preparative form of their active substance.