Microscale Temperature Measurements Within Specific Exposure Systems for Real-Time Cellular Characterization

Abstract

The precise characterization of electromagnetic fields exposure systems requires accurate dosimetry from macroscale down to the cellular level. In this study, two different exposure devices based on microelectrodes arrays for real-time cellular characterization, namely a Micro-Electrode Array (MEA) and an impedancemetry well with interdigitated electrodes were considered. For the first time, the heating induced by the exposure to radiofrequency in the electrodes vicinity was retrieved from the fluorescence intensity of Rhodamine B (Rhod-B), which linearly changes as a function of the temperature variation. Our results confirm that the use of Rhod-B represents a reliable technique to obtain temperature information at the microscopic scale.