STUDYING THE ACUTE TOXICITY AND ANTI-TUMOR ACTIVITY OF THE NEW DRUG COLHAMETIN

Abstract

The aim of the study was to study acute toxicity during intraperitoneal administration of a new antitumor drug "colhametin" (K-2) in mice and rats and its antitumor activity on 2 strains of tumors, including with a smaller number of injections. Material and research methods. Acute toxicity of the K-2 preparation after a single intraperitoneal injection was carried out according to the Litchfield and Wilcoxon method on 30 white outbred mice weighing 20±2g of both sexes and 30 male and female rats weighing 140±10g, 5 animals in each group. The study of antitumor activity was performed on 12 outbred mice and 16 outbred rats with transplanted tumors S-180 (Sarcoma-180) and WC (Walker's sarcoma) which were administered drugs on the 4th day after tumor inoculation 10- and 5-fold. The results were evaluated according to standard criteria: tumor growth inhibition (TGI), body weight and spleen of animals Differences were considered significant at p<0.05. Results. The following data were obtained with a single intraperitoneal injection to mice: the average lethal dose of LD50 is 890 (-150 + 172) mg/kg, LD50 with a single intraperitoneal dose of K-2 preparation in rats, LD50 is 410 (-56 + 61) mg/kg, the values are also determined LD10, LD16 and LD84. The antitumor activity of the drug K-2 on the tumor strain Sarcoma - 180 was high, 99/90%. On WC tumors the effect of K-2 reached 90/91% with 10-fold administration, and 89/89% with 5-fold administration, however, its effect was accompanied by a decrease in hematopoietic parameters. Conclusions. The study of the acute toxicity of the substance of the preparation K-2 showed that this preparation belongs to the IV class of low-toxic compounds. On 2 tumors, the effect of the new drug was high, which did not decrease with a decrease in the number of injections.