Study of Leukopenia Induced by a High Dose of Cyclophosphamide in Mice

Abstract

Abstract: The study was conducted to evaluate the toxic effect of different concentrations of cyclophosphamide (CTX) by inducing leukopenia. Adult female Swiss albino mice weighting (20±4g) arranged in three groups of six animals each housed. A control mice saline PBS solution (group I), mice treated with 200 mg/Kg (4mg/mouse) cyclophosphamide, High dose (group II) and mice treated with treated 100 mg/kg (2mg/mouse) body weight Low dose (group III) for three weeks. Cyclophosphamide causes a significant decrease (P < 0.01) in total leukocyte count (TLC), total erythrocyte count (TEC), and red blood cell distribution (RDW) in all groups compared to the control group of mice. Observed leukopenia inform marked reduced in the absolute numbers of lymphocytes, monocytes, and neutrophils in comparison to the control group of mice. The results indicate that cyclophosphamide alters the blood profile only after high doses while low doses had the least impact on blood pictures. Cyclophosphamide causes a significant increased (P < 0.01) in activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum after CTX treatment with no effect on the serum albumin level compared to the control group. While lower doses had less effect on liver enzymes.