Differential possibilities of classical histological research methods for establishing the genesis of hemorrhages in the human brain substance

Abstract

Verification of the cause of death (CD) from ischemic cerebral infarction (ICI), hemorrhage of traumatic (GTG) and non-traumatic (GNG) genesis eliminates the violent origin of death. Very often it is difficult to diagnose the genesis of hemorrhage only macroscopically when performing an autopsy, so additional material should be selected for forensic histological examination. Aim of the work. To develop forensic criteria for the differentiation of ICI, GTG and GNG of the brain by light microscopy of histological sections of the human brain (HB). Material and methods. For the study were used native sections and stained histological specimens of HB from 110 corpses in the case of: death from ICI – 30 histological specimens (1 group), which were made of 30 speciments stained by the methods of Nissl and Spiel-Mayer; GNG – 30 histological samples (group 2) – 30 speciments, stained similarly to the previous group; GNG – 30 histological samples (group 3) – 30 speciments stained similarly to the previous group. Brain preparations in case of death from coronary heart disease were selected for control – 20 samples (4 groups) – 20 speciments stained by the methods of Nissl and Spiel-Mayer. Results. Analysis of the obtained data of histological examination of morphological changes of tissue elements of the human brain with different genesis of hemorrhage did not reveal stable relationships between changes in the structure of nervous tissue and the cause of hemorrhage. Conclusion. Given the nonspecificity of degenerative changes in the structural elements of the brain, depending on the genesis of hemorrhage, it can be concluded that morphological methods of histological preparations of the brain do not provide accurate and objective information about the genesis of hemorrhage.