Nanopore-spanning lipid bilayer formed by self-spreading method

Zugui Peng, K. Shimba, Yositaka Miyamoto, T. Yagi
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Abstract

Nano-black lipid membranes (nano-BLMs), which are lipid membranes suspended over solid-state nanopores, are used to detect the activities of membrane proteins over longer periods of time than traditional black lipid membranes. However, traditional folding and painting techniques that used to form nano-BLMs have low success rates because they are complicated. Here, we present a simple procedure to form nano-BLMs with a self-spreading method.
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采用自扩散法形成纳米跨孔脂质双分子层
纳米黑色脂质膜(nano-BLMs)是悬浮在固态纳米孔上的脂质膜,用于检测膜蛋白在比传统黑色脂质膜更长的时间内的活性。然而,传统的折叠和绘画技术用于形成纳米blm的成功率很低,因为它们很复杂。在这里,我们提出了一种简单的方法来形成纳米blm的自扩散方法。
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