Decellularization of renal fibrosis by cyclic hydrostatic pressure to restore renal function

Abstract

Natural extracellular matrices (ECM) are currently being studied as an alternative source for organ transplantation or as new solutions to treat end-stage renal disease. As the gap between donors and patients in need of an organ transplant continues to widen, research in regenerative medicine seeks to provide different strategies for treatment Tissue and organ failure is currently one of the biggest health issues our society faces [1]. Arising from disease or trauma, complete treatment typically requires the repair or replace the affected organ However, the unavailability of adequate organs for transplantation to meet the existing demand has resulted in major organ shortage crisis. As a result, there has been a major increase in the number of patients [2]. The stem cell therapy fails because of the toxic environment in the organ, the implanted cells unable to regenerate. Afterwards researchers are trying to prepare the lab made artificial organ in the laboratory with the help of artificial scaffold and the tissue specific stem cells. Then tried for the transplantation of animal organs. To overcome this organ shortage challenge is the use of organ-derived scaffolds obtained via decellularization techniques by removing all viable cells from native tissues and retaining 3D structure of natural extracellular matrix (ECM) and vascular network for regeneration. This is the most adequate method where scaffold thoroughly decellularizes, free of endotoxin or bacterial contamination. An appropriate macro and microstructure to support physiological function, intact vascular tree, specific location cues.