Probiotic Properties of Lactic Acid Bacteria Isolated from Traditional Yoghurt (Dahi) in Sindh Pakistan

A. H. Soomro, Sana Subhopoto, S. A. Sheikh, M. Khaskheli, S. G. Khaskheli, A. Marri, A. B. Baloch
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引用次数: 1

Abstract

The probiotic properties of Lactobacillus isolates of traditional yoghurt were investigated during the study. For that seven species of Lactobacillus were identified phenotypically. Out of 84 isolates,  23 acidophilus, 21 L. casei, 11 L. helveticus, 09 each L. delbrueckii sub sp. bulgaricus and L. delbrueckii sub sp. lactis, 03 L. viridescense and 02 L. plantarum were identified. All of these identified species were screened for antibacterial activity against foodborne pathogens, acid and bile tolerance and antibiotic resistance pattern. The results indicated that L. casei S66, L. delbrueckii sub sp. bulgaricus S65, L. acidophilus S26 and L. plantarum S19 produced antimicrobial substances against the indicator organisms and showed tolerance against acidic pH of 2.5 and survival against the bile salt concentration of 0.1 and 0.2. The L. acidophilus S26 survived the 0.3% bile salt concentration and was resistant to antibiotics ciprofloxacin, kanamycin, penicillin and vancomycin.
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巴基斯坦信德省传统酸奶(Dahi)中乳酸菌的益生菌特性
对传统酸奶中分离乳杆菌的益生菌特性进行了研究。在表型上鉴定出7种乳杆菌。84株分离得到嗜酸乳杆菌23株,干酪乳杆菌21株,helveticus 11株,保加利亚德尔布吕氏乳杆菌亚种和乳酸德尔布吕氏乳杆菌亚种各09株,病毒下降乳杆菌03株,植物乳杆菌02株。对所有鉴定的菌种进行了对食源性致病菌的抗菌活性、酸胆耐受性和抗生素耐药模式的筛选。结果表明,干酪乳杆菌S66、保加利亚德尔布鲁氏杆菌S65、嗜酸乳杆菌S26和植物乳杆菌S19对指示生物产生抑菌物质,对酸性pH为2.5的环境表现出耐受性,对胆盐浓度为0.1和0.2的环境表现出生存能力。嗜酸乳杆菌S26在0.3%胆盐浓度下存活,对环丙沙星、卡那霉素、青霉素和万古霉素耐药。
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