S. Kubota, Y. Iwata, H. Matsuda, K. Imaizumi, S. Miyasaka, M. Yoshino
{"title":"Ultrastructural and Immunohistochemical Changes of Rat Brain after Long-Term Marijuana Inhalation","authors":"S. Kubota, Y. Iwata, H. Matsuda, K. Imaizumi, S. Miyasaka, M. Yoshino","doi":"10.3408/JASTI.5.1","DOIUrl":null,"url":null,"abstract":"Ultrastructural changes of the brain of rats that inhaled marijuana cigarettes for 8 weeks were examined by electron microscopy, simultaneously with immunohistochemical investigation for serotonergic neuronal activities in the nucleus accumbens (NAc), the hippocampus (CA-1) and the nucleus raphe dorsalis (NRD). In the animals that inhaled marijuana, numerous cored and coated vesicles were observed near the well-developed Golgi apparatus of the NRD neurons. The lamellar membranous structure was found frequently in the dendrite, and occasionally in the axon, and the synapse of the marijuana-smoking animals. The serotonin-immunostainability in neurons of the NRD was significantly stronger in the animals that inhaled marijuana than in the non-treated ones. The serotonin-immunostainability in nerve fibers and terminals of the NAc and the CA-1 showed an obvious decrease compared with that of the non-treated animals. These findings suggest that the neurons in the NRD that is the original nucleus of the serotonergic nerve system have become functionally hyperactive by Δ9-tetrahydrocannabinol, but it may induce the dysfunction of serotonin transmission from the original nucleus.","PeriodicalId":134327,"journal":{"name":"Japanese Journal of Science and Technology for Identification","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Science and Technology for Identification","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3408/JASTI.5.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Ultrastructural changes of the brain of rats that inhaled marijuana cigarettes for 8 weeks were examined by electron microscopy, simultaneously with immunohistochemical investigation for serotonergic neuronal activities in the nucleus accumbens (NAc), the hippocampus (CA-1) and the nucleus raphe dorsalis (NRD). In the animals that inhaled marijuana, numerous cored and coated vesicles were observed near the well-developed Golgi apparatus of the NRD neurons. The lamellar membranous structure was found frequently in the dendrite, and occasionally in the axon, and the synapse of the marijuana-smoking animals. The serotonin-immunostainability in neurons of the NRD was significantly stronger in the animals that inhaled marijuana than in the non-treated ones. The serotonin-immunostainability in nerve fibers and terminals of the NAc and the CA-1 showed an obvious decrease compared with that of the non-treated animals. These findings suggest that the neurons in the NRD that is the original nucleus of the serotonergic nerve system have become functionally hyperactive by Δ9-tetrahydrocannabinol, but it may induce the dysfunction of serotonin transmission from the original nucleus.