Ultrastructural and Immunohistochemical Changes of Rat Brain after Long-Term Marijuana Inhalation

S. Kubota, Y. Iwata, H. Matsuda, K. Imaizumi, S. Miyasaka, M. Yoshino
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Abstract

Ultrastructural changes of the brain of rats that inhaled marijuana cigarettes for 8 weeks were examined by electron microscopy, simultaneously with immunohistochemical investigation for serotonergic neuronal activities in the nucleus accumbens (NAc), the hippocampus (CA-1) and the nucleus raphe dorsalis (NRD). In the animals that inhaled marijuana, numerous cored and coated vesicles were observed near the well-developed Golgi apparatus of the NRD neurons. The lamellar membranous structure was found frequently in the dendrite, and occasionally in the axon, and the synapse of the marijuana-smoking animals. The serotonin-immunostainability in neurons of the NRD was significantly stronger in the animals that inhaled marijuana than in the non-treated ones. The serotonin-immunostainability in nerve fibers and terminals of the NAc and the CA-1 showed an obvious decrease compared with that of the non-treated animals. These findings suggest that the neurons in the NRD that is the original nucleus of the serotonergic nerve system have become functionally hyperactive by Δ9-tetrahydrocannabinol, but it may induce the dysfunction of serotonin transmission from the original nucleus.
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长期吸入大麻后大鼠脑超微结构及免疫组织化学的变化
采用电镜观察吸大麻8周大鼠脑超微结构变化,同时免疫组化观察伏隔核(NAc)、海马(CA-1)和背中缝核(NRD) 5 -羟色胺能神经元的活性。在吸入大麻的动物中,在NRD神经元发育良好的高尔基体附近观察到许多有芯和包被的小泡。在抽大麻动物的树突、轴突和突触中多见片层膜结构。吸大麻大鼠NRD神经元的血清素免疫染色明显强于未吸大麻大鼠。NAc和CA-1神经纤维和末梢的血清素免疫染色性与未处理动物相比明显降低。这些发现提示,作为5 -羟色胺能神经系统原核的NRD中的神经元通过Δ9-tetrahydrocannabinol变得功能亢进,但它可能导致5 -羟色胺从原核传递的功能障碍。
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