{"title":"Analysis of Cannabinoids by HPLC Using a Post-Column Reaction with Fast Blue B Salt.","authors":"S. Chinaka, Seishi Tanaka, N. Takayama, K. Ueda","doi":"10.3408/JASTI.5.17","DOIUrl":null,"url":null,"abstract":"Three cannabinoids, D9tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN), were detected with an HPLC method using a post-column reaction with Fast Blue B salt (FBB). The detection limit of THC was 0.1 ng, which is ca. 1000 times more sensitive than TLC using FBB coloration. The calibration curve of THC was linear in the range 0.01-100 mg/ml, and the intermediate precisions of within-run and between-run assays were 0.93% and 1.12%, respectively. The proposed method is highly selective and quantitative.","PeriodicalId":134327,"journal":{"name":"Japanese Journal of Science and Technology for Identification","volume":"60 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Science and Technology for Identification","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3408/JASTI.5.17","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Three cannabinoids, D9tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN), were detected with an HPLC method using a post-column reaction with Fast Blue B salt (FBB). The detection limit of THC was 0.1 ng, which is ca. 1000 times more sensitive than TLC using FBB coloration. The calibration curve of THC was linear in the range 0.01-100 mg/ml, and the intermediate precisions of within-run and between-run assays were 0.93% and 1.12%, respectively. The proposed method is highly selective and quantitative.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
