{"title":"The AE765, AK423, AK692, AO233 and AO234 antibodies recognize human actin by immunofluorescence in HEK cells","authors":"Anna Marchetti","doi":"10.24450/journals/abrep.2022.e759","DOIUrl":null,"url":null,"abstract":"The AE765, AK692, AO233 and AO234 antibodies recognize human actin in PAF-fixed HEK cells by immunofluorescence. Phalloidin, which recognizes polymerized actin, is more adequate to label actin filamentous structures. The AK423 antibody recognizes human actin in methanol-fixed HEK cells, but in these conditions the actin network is poorly preserved.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"7 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibody Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24450/journals/abrep.2022.e759","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The AE765, AK692, AO233 and AO234 antibodies recognize human actin in PAF-fixed HEK cells by immunofluorescence. Phalloidin, which recognizes polymerized actin, is more adequate to label actin filamentous structures. The AK423 antibody recognizes human actin in methanol-fixed HEK cells, but in these conditions the actin network is poorly preserved.
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