Pub Date : 2024-06-10DOI: 10.24450/journals/abrep.2024.e1593
Merina Dhungana, Anna Marchetti
Recombinant anti-V5 tag antibodies AE408 and AY165 were tested for their ability to recognize an overexpressed V5-tagged protein by immunofluorescence. Only AY165 labeled efficiently cells overexpressing a V5-tagged protein.
{"title":"The AY165 antibody recognizes the V5-tag by immunofluorescence","authors":"Merina Dhungana, Anna Marchetti","doi":"10.24450/journals/abrep.2024.e1593","DOIUrl":"https://doi.org/10.24450/journals/abrep.2024.e1593","url":null,"abstract":"Recombinant anti-V5 tag antibodies AE408 and AY165 were tested for their ability to recognize an overexpressed V5-tagged protein by immunofluorescence. Only AY165 labeled efficiently cells overexpressing a V5-tagged protein.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":" 90","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AZ921 recognizes the endogenous USP7 protein by immunofluorescence in HCT116 cells","authors":"Céline Reverdy, J. Rain","doi":"10.24450/journals/abrep.2023.e1433","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1433","url":null,"abstract":"The antibody AZ921 derived from a synthetic VHH recognizes the endogenous USP7 protein in HCT116 cells by immunofluorescence.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139619936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.24450/journals/abrep.2023.e1251
P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca
The recombinant antibodies RB792, RB793, RB794, RB795, RB796 and RB797 recognize by ELISA a synthetic phosphorylated peptide from the human NDC80 protein.
建议抗原RB792 RB793 RB794 RB795 RB797
{"title":"RB792, RB793, RB794, RB795, RB796 and RB797 antibodies recognize a human NDC80 phosphorylated peptide by ELISA","authors":"P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca","doi":"10.24450/journals/abrep.2023.e1251","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1251","url":null,"abstract":"The recombinant antibodies RB792, RB793, RB794, RB795, RB796 and RB797 recognize by ELISA a synthetic phosphorylated peptide from the human NDC80 protein.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126114422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.24450/journals/abrep.2023.e1252
P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca
The recombinant antibodies RB798, RB799, RB800, RB801, RB802, RB803, RB804, RB805, RB806, and RB807 were tested by ELISA for their binding to a synthetic phosphorylated peptide from the human Aurora kinase B (AurKB) protein. Antibodies RB805 and RB806 detected both the phosphorylated and non-phosphorylated peptides, whereas the remaining antibodies selectively recognized the phosphorylated peptide.
{"title":"RB798, RB799, RB800, RB801, RB802, RB803, RB804, RB805, RB806 and RB807 antibodies recognize a human Aurora kinase B (AURKB) phosphorylated peptide by ELISA","authors":"P. Hammel, Keith F. DeLuca, J. Mick, Jennifer G. DeLuca","doi":"10.24450/journals/abrep.2023.e1252","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1252","url":null,"abstract":"The recombinant antibodies RB798, RB799, RB800, RB801, RB802, RB803, RB804, RB805, RB806, and RB807 were tested by ELISA for their binding to a synthetic phosphorylated peptide from the human Aurora kinase B (AurKB) protein. Antibodies RB805 and RB806 detected both the phosphorylated and non-phosphorylated peptides, whereas the remaining antibodies selectively recognized the phosphorylated peptide.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127575497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.24450/journals/abrep.2023.e1227
Lucie Mencier, Lana Moudarres, Gaelle Najand, Laura Roch, Audrey Ruetsche, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Soyan Dawit, Meg-Mai-Ly Diep, Odyssee Ferrillo, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, S. Durual, C. Guilhen, Tania N. Jauslin
The recombinant antibodies AF641, AI954 and AW954 detect different markers of the endoplasmic reticulum by western blot.
重组抗体AF641、AI954和AW954通过western blot检测内质网的不同标记物。
{"title":"AF641, AI954 and AW954 antibodies label markers of the human endoplasmic reticulum by western blot","authors":"Lucie Mencier, Lana Moudarres, Gaelle Najand, Laura Roch, Audrey Ruetsche, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Soyan Dawit, Meg-Mai-Ly Diep, Odyssee Ferrillo, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, S. Durual, C. Guilhen, Tania N. Jauslin","doi":"10.24450/journals/abrep.2023.e1227","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1227","url":null,"abstract":"The recombinant antibodies AF641, AI954 and AW954 detect different markers of the endoplasmic reticulum by western blot.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129740463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.24450/journals/abrep.2023.e1216
B. Bollen Pinto, P. Hammel, Marianne Paolini Bertrand, B. Walder, N. Vuilleumier, O. Hartley
We tested the capacity of antibodies RB733, RB734, RB766, RB767, RB768, RB769, RB770, RB771, RB772 and RB773 to recognize by ELISA three different molecular forms of the human cardiac troponin T isoform 6 (cTnT): an N-terminal peptide (Nter-cTnT), the full-length cTnT protein (FL-cTnT) and the cardiac troponin I-T-C complex (ITC-complex). Our results show that RB734 specifically recognizes the N-terminal region of cTnT as well as the full-length protein alone and the ITC-complex. RB767, RB770, RB771, RB772 and RB773 recognize the full-length cTnT protein alone or in the ITC-complex but not the N-terminal peptide. RB766, RB768 and RB769 recognize the ITC-complex but not cTnT or cardiac troponin I (cTnI) proteins.
{"title":"Recombinant nanobodies detecting the human cardiac troponin proteins by ELISA","authors":"B. Bollen Pinto, P. Hammel, Marianne Paolini Bertrand, B. Walder, N. Vuilleumier, O. Hartley","doi":"10.24450/journals/abrep.2023.e1216","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1216","url":null,"abstract":"We tested the capacity of antibodies RB733, RB734, RB766, RB767, RB768, RB769, RB770, RB771, RB772 and RB773 to recognize by ELISA three different molecular forms of the human cardiac troponin T isoform 6 (cTnT): an N-terminal peptide (Nter-cTnT), the full-length cTnT protein (FL-cTnT) and the cardiac troponin I-T-C complex (ITC-complex). Our results show that RB734 specifically recognizes the N-terminal region of cTnT as well as the full-length protein alone and the ITC-complex. RB767, RB770, RB771, RB772 and RB773 recognize the full-length cTnT protein alone or in the ITC-complex but not the N-terminal peptide. RB766, RB768 and RB769 recognize the ITC-complex but not cTnT or cardiac troponin I (cTnI) proteins.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133136011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AD822, AW199, AW200 and AW201 antibodies against the human Fas receptor (CD95) bind the surface of HEK293 cells as revealed by flow cytometry","authors":"Soyan Dawit, Valerie Schwitzguebel, Wissem Seddiki, Paola Soulie, Nneka Anagbogu, Kenza Bennani, Elise Brun, Hannah Butterworth, Jennifer Carry, Jean-Pierre Aubry-Lachainaye, Cécile Gameiro, S. Durual, Tania N. Jauslin, Audrey Ruetsche, Laura Roch, Meg-Mai-Ly Diep, Odyssee Fer, Maxime Guertler, Catalina Gureu, Margaux Herren, Simon Leyss, Karolina Liborova, Marie-Pierre Maitre, Livia Manghetti, Lucie Mencier, Lana Moudarres, Gaelle Najand, C. Guilhen","doi":"10.24450/journals/abrep.2023.e1143","DOIUrl":"https://doi.org/10.24450/journals/abrep.2023.e1143","url":null,"abstract":"The recombinant antibodies AD822, AW199, AW200 and AW201 bind HEK293 cells expressing the human Fas receptor protein as assessed by flow cytometry.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128684660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：