Bacteria-Adhesive Nitric Oxide-Releasing Graphene Oxide Nanoparticles for MRPA-Infected Wound Healing Therapy

IF 8.2 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY ACS Applied Materials & Interfaces Pub Date : 2022-11-04 DOI:10.1021/acsami.2c13317

Jiafu Cao, Shwe Phyu Hlaing, Juho Lee, Jihyun Kim, Eun Hee Lee, Seok Hee Kang, Suck Won Hong, In-Soo Yoon, Hwayoung Yun, Yunjin Jung and Jin-Wook Yoo*,

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引用次数: 5

Abstract

A bacteria-infected wound can lead to being life-threatening and raises a great economic burden on the patient. Here, we developed polyethylenimine 1.8k (PEI_1.8k) surface modified NO-releasing polyethylenimine 25k (PEI_25k)-functionalized graphene oxide (GO) nanoparticles (GO-PEI_25k/NO-PEI_1.8k NPs) for enhanced antibacterial activity and infected wound healing via binding to the bacterial surface. In vitro antibacterial activity and in vivo wound healing efficacy in an infected wound model were evaluated compared with NO-releasing NPs (GO-PEI_25k/NO NPs). Surface modification with PEI_1.8k can enhance the ability of nanoparticles to adhere to bacteria. GO-PEI_25k/NO-PEI_1.8k NPs released NO in a sustained manner for 48 h and exhibited the highest bactericidal activity (99.99% killing) against methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant Pseudomonas aeruginosa (MRPA) without cytotoxicity to L929 mouse fibroblast cells at 0.1 mg/mL. In the MRPA-infected wound model, GO-PEI_25k/NO-PEI_1.8k NPs showed 87% wound size reduction while GO-PEI_25k/NO NPs showed 23% wound size reduction at 9 days postinjury. Masson trichrome and hematoxylin and eosin staining revealed that GO-PEI_25k/NO-PEI_1.8k NPs enhanced re-epithelialization and collagen deposition, which are comparable to healthy mouse skin tissue. GO-PEI_25k/NO-PEI_1.8k NPs hold promise as effective antibacterial and wound healing agents.

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细菌粘附型一氧化氮释放氧化石墨烯纳米颗粒用于mrpa感染伤口愈合治疗

细菌感染的伤口可能危及生命，并给患者带来巨大的经济负担。在这里，我们开发了聚乙烯亚胺1.8k (PEI1.8k)表面修饰的释放no的聚乙烯亚胺25k (PEI25k)功能化氧化石墨烯纳米颗粒(GO-PEI25k/NO-PEI1.8k NPs)，通过与细菌表面结合来增强抗菌活性和感染伤口愈合。与NO-释放NPs (GO-PEI25k/NO NPs)比较，评价其体外抗菌活性和感染创面模型的体内创面愈合效果。用PEI1.8k对纳米颗粒进行表面改性，可以增强纳米颗粒对细菌的粘附能力。GO-PEI25k/NO- pei1.8 k NPs持续释放NO 48 h，对耐甲氧西林金黄色葡萄球菌(MRSA)和耐多药铜绿假单胞菌(MRPA)表现出最高的杀菌活性(99.99%)，在0.1 mg/mL浓度下对L929小鼠成纤维细胞无细胞毒性。在mrpa感染的创面模型中，GO-PEI25k/NO- pei1.8 k NPs在伤后9天创面大小减少87%，GO-PEI25k/NO NPs创面大小减少23%。马松三色、苏木精和伊红染色显示，GO-PEI25k/NO-PEI1.8k NPs增强了再上皮化和胶原沉积，与健康小鼠皮肤组织相当。GO-PEI25k/NO-PEI1.8k NPs有望成为有效的抗菌和伤口愈合剂。

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来源期刊

ACS Applied Materials & Interfaces 工程技术-材料科学：综合

CiteScore

16.00

自引率

6.30%

发文量

4978

审稿时长

1.8 months

期刊介绍： ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.