EFFECT OF HEAT KILLED BACTERIAL CELL SUPPLIMENTATION FOR GROWTH OF MUSHROOM FUNGUS ( PLEUROTUS EOEUS ) ON MAIZE CROP SUBSTRATE

Abstract

The research was carried out to investigate the cultivation of oyster mushroom ( Pleurotus eoeus ) on maize crop byproducts (maize Stover, maize cob sheath and maize rind). The preliminary test was done to know the effect of heat killed bacterial isolates (Iso-S-1, Iso-S-2, Iso-S-3, Iso-Sh-4, Iso-R-5) on the growth of Pleurotus eoeus in in vitro . Among the heat killed bacterial isolates (Iso-S-3, Iso-R-5) showed the growth stimulatory character on mushroom fungus. So that the selected two bacterial isolates were used for the further study. The Iso-S-3 and Iso-R-5 was grown on nutrient broth then sterilized at 121 0 C for 15 minutes. Then the 100 ml sterilized cell suspended broth was sprayed on the average weight of 2.5-3.0 kg wet base pasteurized maize crop byproducts and paddy straw. This heat killed bacterial cell supplementation (Iso-S-3) enhances the highest growth of Pleurotus eoeus on maize stover substrate and paddy straw compared to the untreated substrates.