ANALISIS RESIDU ANTIBIOTIK TETRASIKLIN PADA DAGING AYAM BROIlER DAN DAGING SAPI

Ary Andini
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引用次数: 2

Abstract

Tetracycline are antibiotics with broad-spectrum that used routinely in veterinary medicine for animal treatment and prevention from infectious diseases. But, consuming antibiotics for long time and exceeding standards can lead to accumulation of antibiotic residues in the body. Based on SNI 01-6366-2000, the maximum residual limit (BMR) of tetracycline antibiotic is 0.1 mg/kg in meat.The purpose of this study was to determine the concentration of tetracycline residues in broiler chicken meat and beef that obtained in East Surabaya namely Manyar market, Sinar Baru market, Ampengan Batu market, Tenggilis Mejoyo market, Krempyeng market, Soponyono market, and Gunung Anyar market. This study used Thin Layer Chromatography (TLC) as qualitative test and Spectrophotometric method as a quantitative test to determine the residual levels of tetracycline antibiotics. This study was conducted on March 15 onto May 2 2019. Based on the study, about 32 broiler chicken and 13 beef samples detected by the TLC method showed that had negative results, there was no containing tetracycline residues in samples. Awhile the results of the spectrophotometric method showed that as many as 31 samples of broiler chicken and 13 beef samples showed that had positive results containing residues of tetracycline antibiotics that exceed of the standard. Based on the results obtained that average concentration of tetracycline in samples of broiler chicken meat about 1,7 mg/kg and 0,8 mg/kg in beef samples. Descriptively,tetracycline residues in broiler chicken meat were higher than beef.
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四环素是兽医学中常用的广谱抗生素,用于动物治疗和预防传染病。但是,长期服用抗生素和超标会导致抗生素残留在体内积累。根据SNI 01-6366-2000,肉类中四环素类抗生素的最大残留限量(BMR)为0.1 mg/kg。本研究的目的是测定泗水东部(Manyar市场、Sinar Baru市场、Ampengan Batu市场、Tenggilis Mejoyo市场、Krempyeng市场、Soponyono市场和Gunung Anyar市场)肉鸡和牛肉中四环素残留的浓度。本研究采用薄层色谱法(TLC)定性检测,分光光度法(光度法)定量检测四环素类抗生素残留水平。该研究于2019年3月15日至5月2日进行。本研究对32份肉鸡和13份牛肉样品进行了薄层色谱检测,结果均为阴性,样品中不含四环素残留。同时,分光光度法检测结果显示,有31份肉鸡样品和13份牛肉样品检测出四环素类抗生素残留量超标。结果表明,肉鸡样品中四环素的平均浓度为1.7 mg/kg,牛肉样品中四环素的平均浓度为0.8 mg/kg。肉用鸡肉中四环素残留量明显高于牛肉。
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