Hyperglycemia is a marker of diabetes mellitus which is generally caused by damaged pancreatic ? cells which interferes with insulin secretion, causing the amount of blood glucose in the body to be uncontrolled. Marsilea crenata is known to contain several phytochemical compounds such as phenolics, flavonoids and steroids which have a positive effect on treating metabolic disorders. This study aims to determine the effect of Marsilea crenata cooking water on blood glucose levels in Mus musculus. The method used to check blood glucose levels is the POCT method. The results obtained in this study indicate that there is a difference in the average blood glucose examination in the treatment group and the control group. The average blood glucose before treatment was found to be 186.1 mg/dl, while measurements at the 4th and 7th hours after therapy with Marsilea crenata boiled water showed results of 166 mg/dl and 155.2 mg/dl. Statistical tests indicated that there was a significant difference or the effect of giving Marsilea crenata boiled water on decreasing blood glucose levels. Keywords : Marsilea crenata, Mus musculus, Blood glucose level, Hyperglycemia
{"title":"PENGARUH AIR REBUSAN DAUN SEMANGGI AIR (Marsilea crenata) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus musculus) HIPERGLIKEMIA","authors":"Yenny Puspa Rini, Gilang Firmanda, Christina Destri Wiwis Wijayanti, Evy Ratnasari Ekawati","doi":"10.51804/jsh.v7i2.14353.63-67","DOIUrl":"https://doi.org/10.51804/jsh.v7i2.14353.63-67","url":null,"abstract":"Hyperglycemia is a marker of diabetes mellitus which is generally caused by damaged pancreatic ? cells which interferes with insulin secretion, causing the amount of blood glucose in the body to be uncontrolled. Marsilea crenata is known to contain several phytochemical compounds such as phenolics, flavonoids and steroids which have a positive effect on treating metabolic disorders. This study aims to determine the effect of Marsilea crenata cooking water on blood glucose levels in Mus musculus. The method used to check blood glucose levels is the POCT method. The results obtained in this study indicate that there is a difference in the average blood glucose examination in the treatment group and the control group. The average blood glucose before treatment was found to be 186.1 mg/dl, while measurements at the 4th and 7th hours after therapy with Marsilea crenata boiled water showed results of 166 mg/dl and 155.2 mg/dl. Statistical tests indicated that there was a significant difference or the effect of giving Marsilea crenata boiled water on decreasing blood glucose levels. Keywords : Marsilea crenata, Mus musculus, Blood glucose level, Hyperglycemia","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.51804/jsh.v7i2.14357.79-84
Novi Ersanto, Adinda Dika Infantria, Titik Sundari, Andita Ayu Mandasari, Siti Nur Husnul Yusmiati
Since specimen collection, preanalytical factors such as time and temperature could affect the peripheral blood smear morphology of erythrocytes, leukocytes, and thrombocytes. Delays are often caused by the limited number of medical personnel so work is not properly organized. This research is an observational qualitative descriptive study to determine the morphology of blood smears immediately checked and delay 24 hours at 2-8 ?. This study aims to determine the evaluation of K3EDTA venous peripheral blood smears immediately checked and delayed 24 hours at 2-8?. From 25 K3EDTA blood samples of Maarif Hasyim Latif Sidoarjo University students, it was found that there were no changes in the morphology of erythrocytes and platelets. Meanwhile, in leukocyte cells, changes occur in the neutrophil segment and stab granules, which is the granules fade, and the leukocyte nuclei can still be identified properly, so that do not affect the examination results. In conclusion, the Evaluation of the K3EDTA Peripheral Blood Smear was immediately checked and delay 24 Hours at the temperature of 2-8 ? has no change, which is still within normal limits. Keywords : Blood Smear, Delayed, K3EDTA, Immediately checked
{"title":"EVALUASI HAPUSAN DARAH TEPI DARAH K3EDTA SEGERA DI PERIKSA DAN DI TUNDA 24 JAM DALAM SUHU 2-8C","authors":"Novi Ersanto, Adinda Dika Infantria, Titik Sundari, Andita Ayu Mandasari, Siti Nur Husnul Yusmiati","doi":"10.51804/jsh.v7i2.14357.79-84","DOIUrl":"https://doi.org/10.51804/jsh.v7i2.14357.79-84","url":null,"abstract":"Since specimen collection, preanalytical factors such as time and temperature could affect the peripheral blood smear morphology of erythrocytes, leukocytes, and thrombocytes. Delays are often caused by the limited number of medical personnel so work is not properly organized. This research is an observational qualitative descriptive study to determine the morphology of blood smears immediately checked and delay 24 hours at 2-8 ?. This study aims to determine the evaluation of K3EDTA venous peripheral blood smears immediately checked and delayed 24 hours at 2-8?. From 25 K3EDTA blood samples of Maarif Hasyim Latif Sidoarjo University students, it was found that there were no changes in the morphology of erythrocytes and platelets. Meanwhile, in leukocyte cells, changes occur in the neutrophil segment and stab granules, which is the granules fade, and the leukocyte nuclei can still be identified properly, so that do not affect the examination results. In conclusion, the Evaluation of the K3EDTA Peripheral Blood Smear was immediately checked and delay 24 Hours at the temperature of 2-8 ? has no change, which is still within normal limits. Keywords : Blood Smear, Delayed, K3EDTA, Immediately checked","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.51804/jsh.v7i2.14334.51-57
Nurcholis Majid
Hematocrit is the total volume of red blood cells in 100 ml of blood expressed as a percentage of blood volume. Hematocrit examination is needed to help diagnose diseases such as anemia and dengue hemorrhagic fever (DHF) to determine the total volume of red blood cells or erythrocytes. The normal value for hematocrit examination is 40-54% for males and 37-47% for females. The purpose of this study was to determine the effect of variations in centrifugation time on hematocrit values using the microhematocrit method. This type of research is quantitative using experimental method with cross sectional design. The method used for hematocrit examination is the microhematocrit method with variations in centrifugation time of 2 minutes, 3 minutes, 4 minutes, and 5 minutes as a control with a speed of 12,000 rpm. The respondents of this study were 35 respondents and the samples of this study were 35 venous blood samples taken from Binawan University students, Medical Laboratory Technology Study Program batch 2021 and 2022. The average result of the 2-minute centrifugation hematocrit value examination is 37.87%, 3-minute centrifugation is 37.57%, 4-minute centrifugation is 37.30%, and 5-minute centrifugation is 37.25%. The Wilcoxon test results show the Sig. (2-tailed) of 2-minute centrifugation compared with 5 minutes is 0.00 (<0.05), 3-minute centrifugation compared with 5 minutes is 0.001 (<0.05), 4-minute centrifugation compared with 5 minutes is 0.560 (>0.05). The results of this study showed that hematocrit values with centrifugation times of 2 minutes and 3 minutes compared to 5 minutes had significant differences in hematocrit values.
{"title":"PENGARUH VARIASI WAKTU SENTRIFUGASI NILAI HEMATOKRIT MENGGUNAKAN METODE MIKROHEMATOKRIT DI UNIVERSITAS BINAWAN","authors":"Nurcholis Majid","doi":"10.51804/jsh.v7i2.14334.51-57","DOIUrl":"https://doi.org/10.51804/jsh.v7i2.14334.51-57","url":null,"abstract":"Hematocrit is the total volume of red blood cells in 100 ml of blood expressed as a percentage of blood volume. Hematocrit examination is needed to help diagnose diseases such as anemia and dengue hemorrhagic fever (DHF) to determine the total volume of red blood cells or erythrocytes. The normal value for hematocrit examination is 40-54% for males and 37-47% for females. The purpose of this study was to determine the effect of variations in centrifugation time on hematocrit values using the microhematocrit method. This type of research is quantitative using experimental method with cross sectional design. The method used for hematocrit examination is the microhematocrit method with variations in centrifugation time of 2 minutes, 3 minutes, 4 minutes, and 5 minutes as a control with a speed of 12,000 rpm. The respondents of this study were 35 respondents and the samples of this study were 35 venous blood samples taken from Binawan University students, Medical Laboratory Technology Study Program batch 2021 and 2022. The average result of the 2-minute centrifugation hematocrit value examination is 37.87%, 3-minute centrifugation is 37.57%, 4-minute centrifugation is 37.30%, and 5-minute centrifugation is 37.25%. The Wilcoxon test results show the Sig. (2-tailed) of 2-minute centrifugation compared with 5 minutes is 0.00 (<0.05), 3-minute centrifugation compared with 5 minutes is 0.001 (<0.05), 4-minute centrifugation compared with 5 minutes is 0.560 (>0.05). The results of this study showed that hematocrit values with centrifugation times of 2 minutes and 3 minutes compared to 5 minutes had significant differences in hematocrit values.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.51804/jsh.v7i2.14355.68-72
Esti Rizkiana Pratiwi, Frida Luthfia Mari, Halik Wijaya, Titik Sundari, Indra Adi Wira Prasetya
Comorbid diabetes mellitus (DM) in pulmonary tuberculosis (TB) patients may increase the risk of death even with or during TB treatment. Immunocompromised conditions due to DM increase the risk of infection and sputum conversion failure. The risk of recurrence in comorbid patient is also higher after treatment. Objective of this research is to describe the comparison of further microscopic examination of pulmonary tuberculosis patients without DM and pulmonary tuberculosis patients with a history of diabetes at the Kedundung Public Health Center. The study was conducted by observing each of 15 TB positive sputum samples accompanied by a history of DM and those without any history of DM 2 months post-treatment on further microscopic examination. Further microscopic examination showed that 100% of sputum samples of TB patients without a history of DM and receiving treatment gave negative results, while sputum samples of TB patients with DM history and receiving treatment, while sputum samples of patients who had received treatment showed results of 2+ by 80% and 3+ by 20% and there were no negative results for TB. These results prove that there is an effect of a history of DM disease on TB treatment outcomes. Keywords : Further microscopic examination, Pulmonary tuberculosis (TB), Diabetes Mellitus (DM)
{"title":"PERBANDINGAN HASIL MIKROSKOPIS LANJUTAN PENDERITA TBC PARU SETELAH PENGOBATAN DENGAN RIWAYAT DM DAN TANPA DM DI PUSKESMAS KEDUNDUNG","authors":"Esti Rizkiana Pratiwi, Frida Luthfia Mari, Halik Wijaya, Titik Sundari, Indra Adi Wira Prasetya","doi":"10.51804/jsh.v7i2.14355.68-72","DOIUrl":"https://doi.org/10.51804/jsh.v7i2.14355.68-72","url":null,"abstract":"Comorbid diabetes mellitus (DM) in pulmonary tuberculosis (TB) patients may increase the risk of death even with or during TB treatment. Immunocompromised conditions due to DM increase the risk of infection and sputum conversion failure. The risk of recurrence in comorbid patient is also higher after treatment. Objective of this research is to describe the comparison of further microscopic examination of pulmonary tuberculosis patients without DM and pulmonary tuberculosis patients with a history of diabetes at the Kedundung Public Health Center. The study was conducted by observing each of 15 TB positive sputum samples accompanied by a history of DM and those without any history of DM 2 months post-treatment on further microscopic examination. Further microscopic examination showed that 100% of sputum samples of TB patients without a history of DM and receiving treatment gave negative results, while sputum samples of TB patients with DM history and receiving treatment, while sputum samples of patients who had received treatment showed results of 2+ by 80% and 3+ by 20% and there were no negative results for TB. These results prove that there is an effect of a history of DM disease on TB treatment outcomes. Keywords : Further microscopic examination, Pulmonary tuberculosis (TB), Diabetes Mellitus (DM)","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.51804/jsh.v7i2.14349.58-62
Tia Novita
Ferritin examination is an important measurement parameter used to measure the presence of iron in the body. Iron deficiency in the body will result in anemia. Anemia that often occurs in pregnant is iron deficiency anemia because the need for iron in pregnant. An increase in erythrocyte sedimentation rate occurs during pregnancy and can also be observed in pregnant. The purpose of this study was to determine the relationship between serum ferritin levels and the value of ESR in second trimester pregnant. This study was conducted at YPK Mandiri Hospital, samples were in the form of medical record data with 92 samples of second trimester pregnant who performed complete hematological examinations and ferritin levels. The results of this study indicate that second trimester pregnant who do complete blood checks and ferritin levels at YPK Mandiri Hospital with ferritin levels ranging from 2 - 219 ng/mL with an average level of 26.31 ng/mL and the value of the Blood Cessation Rate ranging from 5 - 105 mm / hour with an average of 35 mm / hour. This study uses the Spearman Rho test method, obtained a sig value of 0. 962 (>0.05) with a correlation coefficient of 0.005 which means there is no correlation between ferritin levels and the value of the rate of blood absorption in second trimester pregnant.
铁蛋白检测是测定体内铁含量的重要测量参数。体内缺铁会导致贫血。经常发生在孕妇的贫血是缺铁性贫血，因为孕妇需要铁。红细胞沉降率的增加发生在怀孕期间，也可以在怀孕期间观察到。本研究的目的是确定血清铁蛋白水平与妊娠中期血沉值之间的关系。这项研究是在YPK Mandiri医院进行的，样本以医疗记录数据的形式收集了92例妊娠中期孕妇的样本，他们进行了完整的血液学检查和铁蛋白水平检查。本研究结果表明，在YPK Mandiri医院完成血液检查和铁蛋白水平检查的孕中期孕妇，铁蛋白水平范围为2 - 219 ng/mL，平均水平为26.31 ng/mL，停血率范围为5 - 105 mm /小时，平均为35 mm /小时。本研究采用Spearman Rho检验方法，得到sig值为0。962 (>0.05)，相关系数为0.005，说明妊娠中期铁蛋白水平与血吸收率值无相关性。
{"title":"ANALISIS KADAR FERRITIN SERUM DENGAN NILAI LAJU ENDAP DARAH PADA IBU HAMIL TRIMESTER II","authors":"Tia Novita","doi":"10.51804/jsh.v7i2.14349.58-62","DOIUrl":"https://doi.org/10.51804/jsh.v7i2.14349.58-62","url":null,"abstract":"Ferritin examination is an important measurement parameter used to measure the presence of iron in the body. Iron deficiency in the body will result in anemia. Anemia that often occurs in pregnant is iron deficiency anemia because the need for iron in pregnant. An increase in erythrocyte sedimentation rate occurs during pregnancy and can also be observed in pregnant. The purpose of this study was to determine the relationship between serum ferritin levels and the value of ESR in second trimester pregnant. This study was conducted at YPK Mandiri Hospital, samples were in the form of medical record data with 92 samples of second trimester pregnant who performed complete hematological examinations and ferritin levels. The results of this study indicate that second trimester pregnant who do complete blood checks and ferritin levels at YPK Mandiri Hospital with ferritin levels ranging from 2 - 219 ng/mL with an average level of 26.31 ng/mL and the value of the Blood Cessation Rate ranging from 5 - 105 mm / hour with an average of 35 mm / hour. This study uses the Spearman Rho test method, obtained a sig value of 0. 962 (>0.05) with a correlation coefficient of 0.005 which means there is no correlation between ferritin levels and the value of the rate of blood absorption in second trimester pregnant.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.51804/jsh.v7i2.14356.73-78
Elsa Mega Suryani, Yuli Novianti Sunarko, Muhammad Sungging Pradana, Setyo Dwi Santoso, Nadiah Al Batati
Soil is a mineral material that has several unconsolidated forms which are located on the surface of the earth and have undergone several treatments. Soil-Transmitted Helminth (STH) is an intestinal nematode that in its life cycle requires a soil medium for the infective form to mature. worms are moist soil, shady, and protected from sunlight. This study aims to determine whether there is contamination of "Soil-Transmitted Helminth" eggs and what species are present in the rice fields using the Native method. This examination uses a 2% eosin solution. The sample uses paddy fields in Sukodono Village. Sampling at 3 sampling points with 3 types of samples, the total sample is 9 samples of paddy soil. The research was conducted on April 26-28 2021 at the Parasitology Laboratory, Faculty of Health Sciences, Maarif Hasyim Latif University, Sidoarjo, on 9 samples of paddy soil, the results obtained were 3 types of samples, or 33% of the soil contaminated with worm eggs and 6 types of samples or 66% not contaminated with worm eggs. Keywords: Paddy fields, Soil Transmitted Helminth, Native Method
土壤是一种矿物材料，具有几种松散的形式，位于地球表面，经过多次处理。土壤传播蠕虫(STH)是一种肠道线虫，在其生命周期中需要土壤介质来使感染形式成熟。蚯蚓生长在潮湿的土壤里，阴凉，不受阳光照射。本研究旨在利用Native方法确定稻田中是否存在“土壤传播蠕虫”卵的污染以及哪些物种存在。此检查使用2%的伊红溶液。样本使用Sukodono村的稻田。3个采样点采样3种样本，共9个水稻土样本。该研究于2021年4月26日至28日在Sidoarjo Maarif Hasyim Latif大学卫生科学学院寄生虫学实验室对9个水稻土样本进行了研究，获得的结果是3类样本，即33%的土壤被虫卵污染，6类样本，即66%的土壤未被虫卵污染。关键词:水田;土壤传虫;土法
{"title":"IDENTIFIKASI TELUR SOIL TRANSMITTED HELMINT (STH) PADA TANAH PERSAWAHAN DI DESA SUKODONO MENGGUNAKAN METODE NATIF","authors":"Elsa Mega Suryani, Yuli Novianti Sunarko, Muhammad Sungging Pradana, Setyo Dwi Santoso, Nadiah Al Batati","doi":"10.51804/jsh.v7i2.14356.73-78","DOIUrl":"https://doi.org/10.51804/jsh.v7i2.14356.73-78","url":null,"abstract":"Soil is a mineral material that has several unconsolidated forms which are located on the surface of the earth and have undergone several treatments. Soil-Transmitted Helminth (STH) is an intestinal nematode that in its life cycle requires a soil medium for the infective form to mature. worms are moist soil, shady, and protected from sunlight. This study aims to determine whether there is contamination of \"Soil-Transmitted Helminth\" eggs and what species are present in the rice fields using the Native method. This examination uses a 2% eosin solution. The sample uses paddy fields in Sukodono Village. Sampling at 3 sampling points with 3 types of samples, the total sample is 9 samples of paddy soil. The research was conducted on April 26-28 2021 at the Parasitology Laboratory, Faculty of Health Sciences, Maarif Hasyim Latif University, Sidoarjo, on 9 samples of paddy soil, the results obtained were 3 types of samples, or 33% of the soil contaminated with worm eggs and 6 types of samples or 66% not contaminated with worm eggs. Keywords: Paddy fields, Soil Transmitted Helminth, Native Method","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enterobacteriaceae is a group of bacteria that inhabit the digestive tract of humans and animals, capable of causing nosocomial infections and potentially contaminating food products. Dairy products and their analogs are one of the routine food samples tested at the Testing Laboratory of the Balai Besar POM in Surabaya, whose quality and safety must be maintained for consumption by the public. One of the guarantees for the quality of test results is a test method that has been validated and verified. In this study, verification of the Enterobacteriaceae number test method was carried out with reference to SNI ISO 21528-2:2017. The results of the method verification were declared eligible, for the accuracy parameter, which was 102,26%, while for the precision parameter, it was 0,71% I. the sample group that was given the contamination (S+C) and 0,91% in the positive control group (K). It that be concluded that this method can be used in the Testing Laboratory of the Balai Besar POM in Surabaya.Keywords: Method verifications, Enterobacteriaceae, dairy products and their analogs, accuracy, precision.
肠杆菌科是一组栖息在人类和动物消化道的细菌，能够引起医院感染并可能污染食品。乳制品及其类似物是泗水Balai Besar POM检测实验室例行检测的食品样本之一，必须保证其质量和安全，供公众食用。测试结果质量的保证之一是经过验证和验证的测试方法。本研究参照SNI ISO 21528-2:2017对肠杆菌科数量测试方法进行验证。结果表明，该方法的准确度为102.26%，精密度为0.71%，污染样品组(S+C)和阳性对照组(K)的精密度为0.91%，可用于泗水Balai Besar POM检测实验室。关键词:方法验证，肠杆菌科，乳制品及其类似物，准确度，精密度
{"title":"VERIFIKASI METODE UJI ANGKA ENTEROBACTERIACEAE PADA PRODUK SUSU DAN ANALOGNYA SESUAI SNI ISO 21528-2:2017","authors":"Fakhmi Rooslan Hamidi, Dian Indah Octavianingtyas, Inis Nadawati, Evy Ratnasari Ekawati","doi":"10.51804/jsh.v7i1.2068.1-8","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.2068.1-8","url":null,"abstract":"Enterobacteriaceae is a group of bacteria that inhabit the digestive tract of humans and animals, capable of causing nosocomial infections and potentially contaminating food products. Dairy products and their analogs are one of the routine food samples tested at the Testing Laboratory of the Balai Besar POM in Surabaya, whose quality and safety must be maintained for consumption by the public. One of the guarantees for the quality of test results is a test method that has been validated and verified. In this study, verification of the Enterobacteriaceae number test method was carried out with reference to SNI ISO 21528-2:2017. The results of the method verification were declared eligible, for the accuracy parameter, which was 102,26%, while for the precision parameter, it was 0,71% I. the sample group that was given the contamination (S+C) and 0,91% in the positive control group (K). It that be concluded that this method can be used in the Testing Laboratory of the Balai Besar POM in Surabaya.Keywords: Method verifications, Enterobacteriaceae, dairy products and their analogs, accuracy, precision.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135847625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.51804/jsh.v7i1.6846.28-34
Nadiah Al Batati, Dinata Muthmainnata, Evy Ratnasari Ekawati, Setyo Dwi Santoso, Elsa Mega Suryani, Indra Adi Wira Prasetya
This research is based on Mycobacterium tuberculosis infection which can increase the value of erythrocyte sedimentation rate. Where the transmission of these bacteria through the respiratory tract is known as Droplet Infection. The purpose of this study was to find out the effect of one month tuberculosis treatment on the erythrocyte sedimentation rate (ESR) and acid fast bacilli (AFB) examination. This type of research is a laboratory observation study with an accidental sampling technique of 30 samples which was carried out at the Singotrunan Banyuwangi health center for 30 days. The results showed that out of 30 samples examined, it showed that there was an influence of ESR and acid fast bacilli examinations on Tuberculosis patients who had been treated for one month. The conclusion is that there is an influence on Tuberculosis patients on ESR and AFB examinations at the Singotrunan Banyuwangi Health Center. Keywords : Tuberculosis, ESR, AFB
{"title":"Pemeriksaan Basil Tahan Asam dan Laju Endap Darah Sebagai Indikator Keberhasilan Pengobatan Penderita Tuberkulosis Paru di Puskesmas Singotrunan Banyuwangi","authors":"Nadiah Al Batati, Dinata Muthmainnata, Evy Ratnasari Ekawati, Setyo Dwi Santoso, Elsa Mega Suryani, Indra Adi Wira Prasetya","doi":"10.51804/jsh.v7i1.6846.28-34","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.6846.28-34","url":null,"abstract":"This research is based on Mycobacterium tuberculosis infection which can increase the value of erythrocyte sedimentation rate. Where the transmission of these bacteria through the respiratory tract is known as Droplet Infection. The purpose of this study was to find out the effect of one month tuberculosis treatment on the erythrocyte sedimentation rate (ESR) and acid fast bacilli (AFB) examination. This type of research is a laboratory observation study with an accidental sampling technique of 30 samples which was carried out at the Singotrunan Banyuwangi health center for 30 days. The results showed that out of 30 samples examined, it showed that there was an influence of ESR and acid fast bacilli examinations on Tuberculosis patients who had been treated for one month. The conclusion is that there is an influence on Tuberculosis patients on ESR and AFB examinations at the Singotrunan Banyuwangi Health Center. Keywords : Tuberculosis, ESR, AFB","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135847629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.51804/jsh.v7i1.6848.41-46
Maulina Rosida, Budiono Raharjo, Halik Wijaya
Platelets have an important role in hemostasis, namely the formation and stabilization of platelet plugs. There are several methods in the counting of platelets. They can be carried out in a direct and an indirect manner. The direct method can be carried out in an automatic and automatic way. The automatic method uses an hematology analyzer which functions to measure and examine blood cells in blood samples. Counting the number of platelets is done automatically by two methods, namely optical and impedance which have different principles. The optical method is based on cell size and morphology, while impedance is based on cell size only. Hypochrome is a condition where the Hb concentration is less than normal so that the central achromia widens (> 1/3 of the cell) and there is a decrease in the color of the erythrocytes, namely an increase in the diameter of the central pallor above normal so that it looks paler. Microcyte is a condition where the cell size is smaller than normal. The purpose of this study was to determine the difference in the number of platelets in the hematology analyzer using the impedance method and the optical method in the case of microcytic hypochromic anemia (based on erythrocyte index) with a descriptive analytical research design. The test used is the paired t-test with a total sample of 30 samples. The results of platelets using the impedance method have an average of 339.37 * 103 / uL with a standard deviation of 120.294. The results of platelets using the optical method have an average of 314.70 * 103 / uL with a standard deviation of 113.569. A significant value of 0.000 significant value of (sig. < 0.05) can be concluded that there is a difference between the results of platelets on the hematology analyzer using the impedance method and the optical method in cases of microcytic hypochromic anemia (based on erythrocyte index). Keywords : Trombosit, Hipokrom Mikrositer, Hematology analyzer, Impedance, Optik.
{"title":"Analisa Perbandingan Hasil Trombosit Pada Alat Hematologi Analyzer Metode Impedance dan Metode Optik Pada Kasus Anemia Hipokrom Mikrositer (Berdasarkan Indeks Eritrosit)","authors":"Maulina Rosida, Budiono Raharjo, Halik Wijaya","doi":"10.51804/jsh.v7i1.6848.41-46","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.6848.41-46","url":null,"abstract":"Platelets have an important role in hemostasis, namely the formation and stabilization of platelet plugs. There are several methods in the counting of platelets. They can be carried out in a direct and an indirect manner. The direct method can be carried out in an automatic and automatic way. The automatic method uses an hematology analyzer which functions to measure and examine blood cells in blood samples. Counting the number of platelets is done automatically by two methods, namely optical and impedance which have different principles. The optical method is based on cell size and morphology, while impedance is based on cell size only. Hypochrome is a condition where the Hb concentration is less than normal so that the central achromia widens (> 1/3 of the cell) and there is a decrease in the color of the erythrocytes, namely an increase in the diameter of the central pallor above normal so that it looks paler. Microcyte is a condition where the cell size is smaller than normal. The purpose of this study was to determine the difference in the number of platelets in the hematology analyzer using the impedance method and the optical method in the case of microcytic hypochromic anemia (based on erythrocyte index) with a descriptive analytical research design. The test used is the paired t-test with a total sample of 30 samples. The results of platelets using the impedance method have an average of 339.37 * 103 / uL with a standard deviation of 120.294. The results of platelets using the optical method have an average of 314.70 * 103 / uL with a standard deviation of 113.569. A significant value of 0.000 significant value of (sig. < 0.05) can be concluded that there is a difference between the results of platelets on the hematology analyzer using the impedance method and the optical method in cases of microcytic hypochromic anemia (based on erythrocyte index). Keywords : Trombosit, Hipokrom Mikrositer, Hematology analyzer, Impedance, Optik.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135847626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.51804/jsh.v7i1.6732.15-21
Lathifa Azkhiyati, Dheasy Herawati, Setyo Dwi Santoso, Esti Rizkiana Pratiwi, Elsa Mega Suryani
Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation
{"title":"Perbandingan Metode Membran Filter dan Metode Tabung Ganda Terhadap Kandungan Escherichia coli pada Air Bersih","authors":"Lathifa Azkhiyati, Dheasy Herawati, Setyo Dwi Santoso, Esti Rizkiana Pratiwi, Elsa Mega Suryani","doi":"10.51804/jsh.v7i1.6732.15-21","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.6732.15-21","url":null,"abstract":"Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":"468 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135847628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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