Biochemical Parameters and Protein Oxidation Relationship for Hepatitis C Patients and Healthy Ones

Umar Khitab, I. Ullah, A. Zaman, S. Ullah, Muhammad Inam Ullah Malik, Muhammad Shoaib Khan, N. Ullah, Khalid Muhammad, S. Malik, G. Jilani
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Abstract

Globally hepatitis C virus is recognized as one of the basic health issues of liver and result in chronic liver diseases whose diagnosis is difficult and possible only with symptoms until it may leads to liver cancer. DNA damage along with lipids and protein oxidation is caused as result of oxidative stress in hepatitis patients. This work was carried out to search out the oxidation of protein and lipids per-oxidation with relative to DNA damage for hepatitis C patients. Sampling was done in 50 suspected patients among them 20 were positive for HCV, while 30 were negative. With the help of 2-4, dinitrophenyl hydrazine assay quantification of protein carbonyls was done and results showed much increased values than normal. Biochemical parameters of HCV such as complete blood count, triglycerides and cholesterol level were recorded. The results were significant in positive cases than the control group. The study elaborated a new window in the research of chronic HCV patients for severe biochemical parameters alterations, over oxidation of proteins and liver dysfunction. It was therefore concluded that oxidative stress was responsible for increased level of protein oxidation and altered biochemical parameters that leads to damage of liver
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丙型肝炎患者与健康人生化指标及蛋白氧化关系
在全球范围内,丙型肝炎病毒被认为是肝脏的基本健康问题之一,并导致慢性肝病,其诊断困难,可能只有症状,直到可能导致肝癌。肝炎患者氧化应激可引起DNA损伤、脂质和蛋白质氧化。这项工作是为了寻找丙型肝炎患者的蛋白质氧化和脂质过氧化与DNA损伤的关系。对50例疑似患者进行抽样,其中HCV阳性20例,阴性30例。利用2-4对蛋白质羰基进行了二硝基苯肼定量测定,结果显示比正常值高得多。记录全血细胞计数、甘油三酯、胆固醇等HCV生化指标。阳性病例的结果显著高于对照组。该研究为慢性HCV患者严重生化参数改变、蛋白质过度氧化和肝功能障碍的研究开辟了新的窗口。由此得出结论,氧化应激是导致蛋白质氧化水平升高和生化参数改变的原因,从而导致肝脏损伤
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