Glycoconjugate Residues Type in Endometrium Aceh Cattle (Bos indicus) During Estrus Cycle

M. Jalaluddin, T. Budipitojo, D. L. Kusindarta, F. S. Permata, P. Sambodo, H. Hamny, S. Wahyuni, M. Sabri, T. R. Ferasyi, E. Rahmi, A. Awaluddin
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Abstract

The ability of aceh cattle to reproduce in extreme environments is thought to be supported by their reproductive system. This research was conducted to study the reproductive system of Aceh cattle by identifying the types and distribution of glycoconjugates in the endometrium of Aceh cattle at various stages of the estrous cycle. This study used the fifteen pubescent female Acehn cows, the phase of the estrous cycle, and weighing 200-300 kg. Samples were obtained from the slaughterhouse of Banda Aceh City, Aceh Province. The uterus is fixed in Bouin's solution and processed histologically. The uterus is stained with histochemical lectins (Con-A, WGA, RCA, and UEA-I) to detect the type of glycoconjugate. The data obtained are presented in the form of images and analyzed descriptively. The glycoconjugates of D-galactose, L-fucose, D-mannose / D-glucose, and N-acetyl-D-glucosamine can be identified in the endometrium of aceh cattle at various stages of the estrous cycle. The presence of these glycoconjugates is thought to play an important role in the reproductive process that occurs in the endometrium of aceh cattle during the estrous cycle.
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亚齐牛(Bos indicus)发情期子宫内膜糖缀合物残基类型
亚齐牛在极端环境中繁殖的能力被认为是由它们的生殖系统支持的。本研究通过确定亚齐牛在发情周期的不同阶段子宫内膜中糖缀合物的类型和分布来研究亚齐牛的生殖系统。本研究选取了15头处于发情周期、体重200-300公斤的雌性亚齐奶牛。样本来自亚齐省班达亚齐市的屠宰场。子宫固定在Bouin溶液中并进行组织学处理。子宫用组织化学凝集素(Con-A, WGA, RCA和UEA-I)染色以检测糖结合物的类型。所获得的数据以图像的形式呈现,并进行描述性分析。d -半乳糖、L-焦糖、d -甘露糖/ d -葡萄糖和n -乙酰- d -氨基葡萄糖的糖缀合物可以在亚齐牛发情周期的不同阶段的子宫内膜中被鉴定出来。这些糖缀合物的存在被认为在亚齐牛发情周期子宫内膜的生殖过程中起重要作用。
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