Preliminary study in genetic polymorphism of hOGG1 and risk factor for thyroid cancer in Indonesia

Abstract

The human 8-oxoguanine DNA N-glycosylase-1 (hOGG1) is the most important DNA repair enzyme in base excision repair (BER) pathways and has been reported to have a relationship with the risk of developing various cancers. The study was aimed to assess the genetic polymorphism of hOGG1 as a risk factor for thyroid cancer. A total of 19 participants were enrolled in this study, consisted of ten thyroid cancer patients as a case group and nine non-cancer patients as a control group. Examination of hOGG1 genotype was carried out by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method and statistical analysis using a Chi-square test. In this study, we found the frequency of hOGG1 genetic polymorphism was not significantly different between cancer patients and control groups and we predict that the polymorphism was not a risk factor for cancer (P>0.05). Results showed the frequency of G allele (mutant) was 0.5 in the case group and 0.33 in the control group. We found that frequency of hOGG1 genetic polymorphism was not significantly different between cancer patients and control groups and this work predicts that the polymorphism was not a risk factor for thyroid cancer. In further studies, it is necessary to assess genetic polymorphisms in populations with controlled non-genetic factors, such as diet, lifestyle, and environmental factor. The relatively small sample size must be considered as a limitation of the study, and thus further research is needed in different populations with larger sample sizes.