USE OF BEET ROOT FOR CULTURING GRAM NEGATIVE BACTERIA IN LABORATORY

Abstract

Microbes were grown in the laboratory for different purposes, by supporting their needs for growth .Nutrient Agar is a universal medium used for growing a wide spectrum of bacteria. The needing to make a medium which could replace the used commercial media by using materials which is available at the local market and cheap ,especially using these media at scientific search laboratories cost so much due to high needs and use. This led to think about using grains and plant roots as a substitute materials in order to prepare new media to cultivate bacteria. This study use Beet root to prepare a medium to replace the traditional ready media which used to cultivate bacteria. Detection the growth of Gram negative bacteria on Beet root Agar and Broth was done , preparation of Differential Beet Root media by adding lactose, bile salts and neutral red stain. The Differential Beet root media efficacy in growth of some Gram negative and positive pathogenic bacteria werenot significant at 0.05, if compared with the Nutrient and MacConkey media . Differentiation between lactose fermenter and non-lactose fermenter was done on differential Beet root media. It had the ability to inhibit the growth of Staphylococcus aureus in comparison with other culture media at 0.05 level. This study showed the possibility of using available , cheap and simple materials as a medium which could replace the traditional ready media , after adding nourishing and mineral salt materials which was convenient for bacteria without any change in metabolism or the morphology