Azathioprine-induced Myelodysplasia Mimicking Myelodysplastic Syndrome

Abstract

An 81-year-old man suffering from autoimmune hepatitis, who had been on prednisolone (10–20 mg/day) and azathioprine (AZA) (50 mg/day) for about 5 months, was diagnosed with macrocytic anemia during a routine examination. Before treatment with prednisolone and AZA, complete blood count revealed a white blood cell count of 9,780/μL, hemoglobin level of 11.7 g/dL with a mean corpuscular volume of 91.9 fL (normal range, 80–99 fL), and platelet count of 18.4×10/μL. Five months after treatment with prednisolone and AZA, complete blood count revealed a white blood cell count of 6,180/μL, hemoglobin level of 7.2 g/dL with a mean corpuscular volume of 109.1 fL, and platelet count of 17.5×10/μL. Serological tests revealed iron level of 116 μg/dL (normal range, 40–190 μg/ dL), free thyroxine level of 1.10 ng/dL (normal range, 0.9–1.70 ng/dL), vitamin B12 level of 462 pg/mL (normal range, 233–914 pg/mL), folic acid level of 7.4 ng/mL (normal range, 3.6–12.9 ng/mL), and anti-DNA antibody (Ab) level of <1.7 IU/mL (normal value, <6.0 IU/mL). The results of tests for antivirus immunoglobulin M (IgM) Ab were negative for antiparvovirus B19 IgM Ab, anti-Epstein-Barr virus capsid antigen IgM Ab, and anti-cytomegalovirus IgM Ab. Bone marrow aspiration revealed a normocellular bone marrow. The aspiration smear revealed a neutrophil with hypersegmentation (Fig. 1), an erythroblast with a bilobulated nucleus (Fig. 2A), an erythroblast with abundant cytoplasm and a multilobulated nucleus (Fig. 2B), an erythroblast with a multilobulated nucleus and HowellJolly bodies (Fig. 2C), an erythrocyte with abundant cytoplasm and Howell-Jolly bodies (Fig. 2D), and a megakaryocyte with separated nuclei (Fig. 3). However, no chromosomal aberrations were observed. Based on these findings, vitamin B12 or folic acid deficiency, viral infections, and systemic lupus erythematosus that led to anemia with myelodysplasia were not diagnosed. Considering AZA-induced hematopoietic disorder, AZA treatment was discontinued, leading to normalization of macrocytic Images and Solution Ewha Med J 2019;42(3):46-47 https://doi.org/10.12771/emj.2019.42.3.46 eISSN 2234-2591