In vitro transfer of N-acetylglucosamine to endogenous glycoprotein acceptors catalyzed by the nucleus and the cytoplasmic membranes prepared from L1210 cells.
{"title":"In vitro transfer of N-acetylglucosamine to endogenous glycoprotein acceptors catalyzed by the nucleus and the cytoplasmic membranes prepared from L1210 cells.","authors":"J Frot-Coutaz, A Degiuli, R Létoublon, J Vila","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The non-nuclear membranes and the nuclei prepared from L1210 cells catalyze the in vitro transfer of N-acetyl(14C)glucosamine from UDP-N-acetyl(14C)glucosamine to endogenous glycoprotein acceptors. Adequate analysis of these acceptors have demonstrated that the nucleus has its own N-acetylglucosaminyltransferase system that leads to the formation of N-N'-diacetylchitobiosylated proteins.</p>","PeriodicalId":8778,"journal":{"name":"Biochemistry international","volume":"28 5","pages":"905-20"},"PeriodicalIF":0.0000,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry international","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The non-nuclear membranes and the nuclei prepared from L1210 cells catalyze the in vitro transfer of N-acetyl(14C)glucosamine from UDP-N-acetyl(14C)glucosamine to endogenous glycoprotein acceptors. Adequate analysis of these acceptors have demonstrated that the nucleus has its own N-acetylglucosaminyltransferase system that leads to the formation of N-N'-diacetylchitobiosylated proteins.
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