The study of phenolic compounds in Canna lily flowers using HPLC

Svetlana Tymofieieva, O. Kyslychenko, I. Zhuravel
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Abstract

Purpose . The purpose of our work was the research of phenolic compounds in canna lily ( Canna x hybrida Hort.) flowers for a further detailed study of canna lily plant raw material phytochemical composition. Materials and methods . Canna lily flowers of red color collected in Ukraine in summer 2014-2016 were chosen as the object of the study. Ethanol extracts obtained from dried flowers were used for the research. The extracts were analyzed using High Performance Liquid Chromatography (HPLC) technique on a liquid chromatograph equipped by diode-array detector Shimadzu HPLC-system, ser 20. HPLC technique was used to detect phenolic compounds in canna lily flowers. Results and conclusions . Rutin, neochlorogenic, chlorogenic, caffeic and rosemarinic acids were identified in canna lily flowers. The obtained experimental data can be used in the development of the standardization parameters of canna lily flowers.
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HPLC法研究美人蕉百合花中酚类化合物
目的。本研究的目的是研究美人蕉(canna x hybrida Hort.)花中的酚类化合物,为进一步详细研究美人蕉植物原料的植物化学成分奠定基础。材料和方法。选取2014-2016年夏季在乌克兰采集的红色美人蕉百合花作为研究对象。以干花为原料提取乙醇提取物进行研究。采用高效液相色谱(HPLC)技术,在配备岛津(Shimadzu) se20型二极管阵列检测器的液相色谱仪上对提取物进行分析。采用高效液相色谱法测定美人蕉百合花中酚类化合物的含量。结果和结论。从美人蕉百合花中鉴定出芦丁酸、新绿原酸、绿原酸、咖啡酸和迷迭香酸。所得实验数据可用于美人蕉百合花标准化参数的制定。
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